Single Cell Analysis, 1st ed. 2024 Methods and Protocols Methods in Molecular Biology Series, Vol. 2752

This volume explores the latest advancements and techniques used to study cell analysis, their capabilities, and the type of results that can be obtained. The chapters in this book cover topics such as FACS; fluorescence microscopy; organic spectroscopy such as MALDI; inorganic spectroscopy such as ICP-MS; and sequencing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and practical, Single Cell Analysis: Methods and Protocols is a valuable tool for any researcher interested in learning more about this important and developing field. 

Acknowledgement…

Preface…

Table of Contents…

Contributing Authors…

1. Single Cell Isolation from Surgically Resected Tissue via Mechanical Dissociation using TissueGrinder

Prama Pallavi and Stefan Scheuermann

2. Circulating Tumor Cell Enrichment and Single Cell Isolation Combining the CellSearch® and DEPArray™ Systems

Cäcilia Köstler, Bernhard Polzer, and Barbara Alberter

3. Isolation of Viable Epithelial and Mesenchymal Circulating Tumor Cells from Breast Cancer Patients

Justyna Topa, Anna J. Żaczek, and Aleksandra Markiewicz

4. Single Cell Recovery from Tumor Cell Xenotransplanted Zebrafish Embryos for the Study of Metastasis Initiating Cells

Pablo Hurtado, Inés Martínez-Pena, and Roberto Piñeiro

5. Isolation of Single Circulating Tumor Cells using VyCAP Puncher System

Thais Pereira-Veiga, Bianca Behrens, Joska J. Broekmaat, Lisa Oomens, Michiel Stevens, Arjan G.J. Tibbe, Nikolas Stoecklein, Laura Muinelo-Romay, Roberto Piñeiro, and Clotilde Costa

6. Simultaneous Isolation and Amplification of mRNA and Genomic DNA of a Single Cell

Miodrag Gužvić

7. Isolation and Genomic Analysis of Circulating Tumor Cell Clusters in Cancer Patients

Carolina Reduzzi, Marta Vismara, Thomas Schamberger, Marco Silvestri, Rosita Motta, Bernhard M. Polzer, and Vera Cappelletti

8. Establishing Single Cell Clones from In Vitro Cultured Circulating Tumor Cells

Teng Teng and Min Yu

9. Immunofluorescence Combined with Single-Molecule RNA Fluorescence In Situ Hybridization for Concurrent Detection of Proteins and Transcripts in Stress Granules

Jakub Kochan and Mateusz Wawro

10. Highly Multiplexed and Simultaneous Characterization of Protein and RNA in Single Cells by Flow or Mass Cytometry Platforms using Proximity Ligation Assay for RNA

Andrew D. Duckworth, Joseph R. Slupsky, and Nagesh Kalakonda

11. Array-Based Comparative Genomic Hybridization for the Detection of Copy Number Alterations in Single Cells

Giancarlo Feliciello, Zbigniew Tadeusz Czyz, and Bernhard Polzer

12. Single Cell MicroRNA Sequencing Library Preparation

Sarah M. Hücker and Stefan Kirsch

13. Immunoblot Analysis from Single Cells using Milo™ Single-Cell Western Platform

Prashant V. Thakkar

Amy J. Managh and Calum Greenhalgh

15. Patch-seq: Multimodal Profiling of Single-Cell Morphology, Electrophysiology, and Gene Expression

Cathryn R. Cadwell and Andreas S. Tolias

Michail Yekelchyk, Xiang Li, Stefan Guenther, and Thomas Braun

Subject Index List…