Single Cell Metabolism, 1st ed. 2020 Methods and Protocols Methods in Molecular Biology Series, Vol. 2064

This volume explores the latest techniques and workflow for the analysis of single cells metabolism. The chapters in this book cover topics such as the development of mass spectrometry-based single cell approaches, Pico-ESI-MS for single-cell metabolomics analysis; laser capture microdissection; ambient single cell metabolite profile (DESI and LAESI); and MALDI-MS methodology, quantum dots for quantitative cytology to study metabolic heterogeneity of single cells. Written in the highly successful Methods in Molecular Biology series format, the chapters consist of  introductions to the topic, lists of the necessary materials and reagents, step-by-step guidelines, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Comprehensive and authoritative, Single Cell Metabolism: Methods and Protocols is a valuable resource for any researcher and scientist interested in learning more about this field. 

Preface…

Table of Contents…

Contributing Authors…

1. Single Cell Metabolomics by Mass Spectrometry

Bindesh Shrestha

2. Identification of Metabolites in Single Cells by Ion Mobility Separation and Mass Spectrometry

Linwen Zhang, Linda L. Allworth, and Akos Vertes

3. Analysis of Lipids in Single Cells and Organelles using Nanomanipulation-Coupled Mass Spectrometry

Mandy S. Phelps and Guido F. Verbeck

4. Development of Pico-ESI-MS for Single-Cell Metabolomics Analysis

5. Single-Probe Mass Spectrometry Analysis of Metabolites in Single Cells

Ning Pan, Wei Rao, and Zhibo Yang

6. Applications of MicroArrays for Mass Spectrometry (MAMS) in Single Cell Metabolomics

Alfredo J. Ibáñez and Ales Svatos

7. Laser Capture Microdissection – Liquid Vortex Capture – Mass Spectrometry Metabolic Profiling of Single Onion Epidermis and Microalgae Cells

John F. Cahill and Vilmos Kertesz

8. Single Cell Analysis by High-Resolution Atmospheric-Pressure MALDI MS Imaging

Dhaka Ram Bhandari, Giulia Coliva, Maria Fedorova, and Bernhard Spengler

9. A MALDI-MS Methodology for Studying Metabolic Heterogeneity of Single Cells in a Population

Jasmin Krismer, Jens Sobek, Robert F. Steinhoff, Rolf Brönnimann, Martin Pabst, and Renato Zenobi

10. Sample Preparation and Analysis of Single Cells using High Performance MALDI FTICR Mass Spectrometry

Bo Yang, Tina Tsui, Richard M. Caprioli, and Jeremy L. Norris

11. Toward Single Cell Molecular Imaging by Matrix-Free Nanophotonic Laser Desorption Ionization Mass Spectrometry

Sylwia A. Stopka and Akos Vertes

12. Compact Quantum Dots for Quantitative Cytology

Phuong Le, Shweta Chitoor, Chunlai Tu, Sung Jun Lim, and Andrew M. Smith

13. Ambient Lipidomic Analysis of Single Mammalian Oocytes and Preimplantation Embryos using Desorption Electrospray Ionization (DESI) Mass Spectrometry

Christina R. Ferreira, Valentina Pirro, Alan K. Jarmusch, Clint M. Alfaro, and R. Graham Cooks

14. Spatial Mapping of Cellular Metabolites using DESI Ion Mobility Mass Spectrometry

Anthony Midey, Hernando Olivos, and Bindesh Shrestha

15. Open-Source Software Tools, Databases, and Resources for Single Cell and Single Cell-Type Metabolomics

Biswapriya B. Misra

16. Ten Major Future Challenges in Single Cell Metabolomics

Bindesh Shrestha