Mitochondria, 1st ed. 2022 Methods and Protocols Methods in Molecular Biology Series, Vol. 2497
This volume details comprehensive protocols and methodologies to assess mitochondrial bioenergetics and dynamics in different tissues and cells involving health and pathological states. Chapters guide readers through methods for assessment of the energy metabolism including Oxygen Consumption Rate (OCR), mitochondrial membrane potential, and measuring mitochondrial Ca2+ handling, and ROS emission. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting, and systematic reproducible protocols.
Authoritative and cutting-edge, Mitochondria: Methods and Protocols aims to be a foundation for future studies and to be a source of inspiration for new investigations in the field.
1. Fast Determination of Mitochondrial Metabolism and Respiratory Complex Activity in Permeabilized and Intact Cells
Kareem A. Heslop, Amandine Rovini, Monika Gooz, and Eduardo N. Maldonado
2. Unbiased Millivolts Assay of Mitochondrial Membrane Potential in Intact Cells
Chad A Lernerand Akos A Gerencser
3. Rotenone Decreases Ischemia-induced Injury by Inhibiting Mitochondrial Permeability Transition – A study in Brain
Ramune Morkuniene, Evelina Rekuviene and Dalia M. Kopustinskiene
4. Assessment of Mitochondrial Complex II and III Activity In Brain Sections: a Histoenzymological Technique
Rubina Roy, Rajib Paul, Pallab Bhattacharyya, and Anupom Borah
5. Measurement of mitochondrial (dys)function in cellular systems using Electron Paramagnetic Resonance (EPR): oxygen consumption rate and superoxide production
Donatienne d’Hose and Bernard Gallez
6. Mitochondrial Calcium Handling in Isolated Mitochondria from a Guinea Pig Heart
Jyotsna Mishra and Amadou K. S. Camara
7. Characterizing the Electron Transport Chain---Structural Approach
Yidong Bai8. Characterizing the electron transport chain--functional approach using Extracellular Flux Analyzer on mouse tissue samples
Ting Liang, Jay Dunn, Xin Zou, Bijaya Nayak, Yuji Ikeno, Lihong Fan, and Yidong Bai
9. Simultaneous Acquisition of Mitochondrial Calcium Retention Capacity and Swelling to Measure Permeability Transition Sensitivity
Arielys M. Mendozaand Jason Karch
10. Measuring mitochondrial Function: From organelle to organism
Matthew T. Lewis, Yan Levitsky, Jason N. Bazil, and Robert W. Wiseman
11. Mitochondrial Toxicity of Organic Arsenicals
Yu-Jiao Liuand Yi Liu
12. Measurements of Mitochondrial Respiration in Intact Cells, Permeabilized Cells, And Isolated Tissue Mitochondria using the Seahorse XF Analyzer
Jessica Pfleger
13. Monitoring mitochondrial morphology and respiration in Doxorubicin-induced cardiomyopathy
Chowdhury S Abdullah, Richa Aishwarya, Mahboob Morshed, Naznin Sultana Remex, Sumitra Miriyala, Manikandan Panchatcharam, and Shenuarin Bhuiyan
14. Creation of yeast models for evaluating the pathogenicity of mutations in the human mitochondrial gene MT-ATP6 and discovering therapeutic molecules
Tribouillard-Tanvier Déborah, Dautant Alain, Godard François, Panja Chiranjit, di Rago Jean-Paul and Kucharczyk Roza
15. In vivo analysis of mitochondrial protein synthesis in Saccharomyces cerevisiae mitochondrial tRNA mutants
Arianna Montanari
16. Visualizing mitochondrial importability of a protein using the yeast Bi-genomic Mitochondrial-Split-GFP strain and an ordinary fluorescence microscope
Marine Hemmerle, Bruno Senger, Roza Kucharczyk and Hubert D. Becker
17. Analysis of Mitochondrial Performance in Lymphocytes using Fluorescent Life--Time Imaging Microscopy
Meha Patel, Javier Manzella--Lapeira, and Munir Akkaya
18. Monitoring mitochondrial perturbations during infection
Varnesh Tiku and Man-Wah Tan
19. Measuring the Mitochondrial Ubiquinone (Q) Pool Redox State In Isolated Respiring Mitochondria
Marten Szibor, Estelle Heyne, Carlo Viscomi, and Anthony L. Moore
20. Experimental Setup For Investigation Of Acute Mitochondrial Oxygen Sensing In Primary Cells
Fenja Knoepp1, Norbert Weissmann, Natascha Sommer, and Marten Szibor
21. Assessing the redox status of mitochondria through the NADH/FAD+ ratio in intact cells
Haoyu Chi, Gauri Bhosaleand Michael R. Duchen
22. Monitoring mitochondrial membrane potential in live cells using time-lapse fluorescence imaging
Gabriel Esteban Valdebenito and Michael R. Duchen
23. Investigating mitochondrial Ca2+ dynamics in isolated mitochondria and intact cells: Application of fluorescent dyes and genetic reporters
Gauri Bhosale and Michael R. Duchen
24. A plate reader-based measurement of the cellular ROS production using Dihydroethidium and MitoSOX
Chih-Yao Chung and Michael R. Duchen
25. Analysis of organisation and activity of mitochondrial respiratory chain complexes in primary fibroblasts using blue native PAGE
Kritarth Singh and Michael R. Duchen
26. Multiplexing Seahorse XFe24 and ImageXpress® Nano platforms for comprehensive evaluation of mitochondrial bioenergetic profile and neuronal morphology
Smijin K. Soman, Maryann Swain, and Ruben K. Dagda
27. Computational Modelling of Mitochondria to Understand the Dynamics of Oxidative Stress
Rashmi Kumar and Mohsin S. Jafri
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Date de parution : 07-2023
Ouvrage de 425 p.
17.8x25.4 cm
Date de parution : 07-2022
Ouvrage de 425 p.
17.8x25.4 cm
Thème de Mitochondria :
Mots-clés :
Biogenesis; yeast; cardiolipin; mitochondrial bioenergetics; confocal microscopy
