Mitochondrial Regulation, Softcover reprint of the original 1st ed. 2015 Methods and Protocols Methods in Molecular Biology Series, Vol. 1241
In this volumeexpert researchers in the field detail the many methods for further research into regulation of mitochondrial function. Chapters focus on mitochondria with other cellular components, discussing how these interactions influence the dynamics of mitochondrial structure and biogenesis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Mitochondrial Regulation: Methods and Protocols seeks to aid advanced undergraduates, graduates, postgraduates, and beginning researchers in the areas of molecular and cellular biology, biochemistry and bioenergetics.
1. Isolation of Crud Mitochondrial Fraction from Cells
Mariusz R. Wieckowski and Lech Wojtczak
2. Isolation of Mitochondria from Liver and Extraction of Total RNA and Protein – Analyses of MicroRNA and Protein Expressions
Pedro M. Borralho, Clifford J. Steer, and Cecília M. P. Rodrigues
3. PCR Based Determination of Mitochondrial DNA Copy Number in Multiple Species
Rooney JP, Ryde IT, Sanders LH, Howlett EH, Colton MD, Germ KE, Mayer GD, Greenamyre JT, and Meyer JN
4. NAD+ Content and its Role in Mitochondria
Wei Li and Anthony A. Sauve
5. Measuring PGC-1α and its Acetylation Status in Mouse Primary Myotubes
Ana P. Gomes and David A. Sinclair
6. Measurement of Mitochondrial Oxygen Consumption Rates in Mouse Primary Neurons and Astrocytes
Sofia M. Ribeiro, Alfredo Giménez-Cassina, and Nika N. Danial
7. Determination Of Oxidative Phosphorylation Complexes Activities
João S. Teodoro, Carlos M. Palmeira, and Anabela P. Rolo
8. Histoenzymatic Methods for Visualization of the Activity of Individual Mitochondrial Respiratory Chain Complexes in the Muscle Biopsies from Patients with Mitochondrial Defects
Agnieszka Karkucinska-Wieckowska, Maciej Pronicki, and Mariusz R. Wieckowski
9. A Method Aimed at Assessing the Functional Consequences of the Supramolecular Organization of the Respiratory Electron Transfer Chain by Time-resolved Studies
Fabrice Rappaport
10. BN-PAGE-based Approach to Study Thyroid Hormones and Mitochondrial Function
Elena Silvestri, Assunta Lombardi, Federica Cioffi , and Fernando Goglia
11. Detection of UCP1 Protein and Measurements of Dependent GDP-Sensitive Proton Leak in Non-Phosphorylating Thymus Mitochondria
Kieran J. Clarke, Audrey M. Carroll, Gemma O’Brien & Richard K. Porter
12. Exploring Liver Mitochondrial Function By 13c-Stable Isotope Breath Tests. Implications In Clinical Biochemistry
Ignazio Grattagliano, Leonilde Bonfrate, Michele Lorusso, Luigi Castorani, Ornella de Bari, and Piero Portincasa
13. Following Mitochondria Dynamism: Confocal Analysis of The Organelle Morphology
F. R. Mariotti, M. Corrado, and S. Campello
14. Analysis of Pro-apoptotic Protein Trafficking to and From MitochondriaIgnacio Vega-Naredo, Teresa Cunha-Oliveira, Teresa Serafim, Vilma Sardao, and Paulo J. Oliveira
15. Mitophagy and Mitochondrial Balance
Simone Patergnani and Paolo Pinton
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Includes supplementary material: sn.pub/extras
Ouvrage de 201 p.
17.8x25.4 cm
Date de parution : 10-2014
Ouvrage de 201 p.
17.8x25.4 cm