Intracellular Thermometry with Fluorescent Molecular Thermometers
Auteur : Uchiyama Seiichi
Understand a vital new bioanalytical technique with this comprehensive introduction to measuring temperature on the cellular scale
Most organisms have highly controlled body temperatures, fluctuations in which are therefore sensitive indicators of changes in body function. In recent years, the development of fluorescent molecular thermometers and related intracellular temperature probes has enabled researchers to track these fluctuations at the cellular rather than the organismic level, opening up a whole new field of study in cell and molecular biology.
Intracellular Thermometry with Fluorescent Molecular Thermometers provides bioanalytical researchers with an introduction to these technologies and their current and future applications. Starting off with a discussion of temperature as a key factor in biological regulation, it provides an authoritative overview of available fluorescent temperature probes, their characteristics and potential applications.
Intracellular Thermometry with Fluorescent Molecular Thermometers readers will also find:
- Step by step instructions for constructing an intracellular thermometry experiment and validating results
- Comprehensive discussion of existing applications
- A vision for the future development of thermal biology as an independent discipline
Authored by a pioneer in the field of intracellular thermometry, Intracellular Thermometry with Fluorescent Molecular Thermometers is ideal for researchers in analytical chemistry, cell biology, molecular biology, biophysics, or any related subjects.
Definition of temperature
Body temperature
Effects of environmental temperature on biological functions
FLUORESCENT PROBES FOR MEASURING TEMPERATURE
Physical chemistry of fluorescence
Fluorescent molecular thermometers
- small organic molecules
- organometallic complexes
- inorganic materials
- macromolecules (including fluorescent proteins)
INTRACELLULAR THERMOMETRY
Endogenous thermogenesis
Effects of external stimuli on intracellular temperature
Comparable non-fluorescent technique in intracellular thermometry
Validation of intracellular thermometry
Relationship between intracellular temperature and biological events
BEYOND INTRACELLULAR THERMOMETRY
"Thermal biology"
In vivo internal thermometry of living species
Future perspectives
Seiichi Uchiyama, PhD, is an Assistant Professor at the University of Tokyo, Japan. He received his academic degrees in Pharmacy from the University of Tokyo. Following this, he was a postdoctoral researcher at Nara Women’s University and Queen’s University of Belfast. His research interests include analytical and photophysical chemistry and the development of fluorescent sensors based on novel functional mechanisms.
Date de parution : 04-2024
17x24.4 cm