Viral Applications of Green Fluorescent Protein, 2009 Methods and Protocols Methods in Molecular Biology Series, Vol. 515

Using Fluorescent Proteins to Study Poxvirus Morphogenesis.- Molecular Imaging of Human Embryonic Stem Cells.- Use of a GFP-PML-Expressing Cell Line as a Biosensor for Human Cytomegalovirus Infection.- Dual-Color Imaging of Tumor Angiogenesis.- Use of GFP to Analyze Morphology, Connectivity, and Function of Cells in the Central Nervous System.- Expression of EGFP by Adenovirus-Mediated Gene Transfer in the Central Nervous System.- Adenovirus-Mediated Delivery of Short Hairpin RNA (shRNA) Mediates Efficient Gene Silencing in Terminally Differentiated Cardiac Myocytes.- Microscopic Analysis of Adenoviral Decontamination Using GFP Adenovirus with Comparable Sensitivity to Flow Cytometry.- Production of Multicistronic HIV-1 Based Lentiviral Vectors.- Using an EGFPmeter to Evaluate the Lentiviral Vector Production: Tricks and Traps.- Use of a Macrophage-Tropic GFP-Tagged Human Immunodeficiency Virus Type 1 (HIV-1) to Study Viral Reservoirs.- GFP-Lentiviral Vectors Targeting for NeuroAIDS.- Construction of Cell-Type Specific Promoter Lentiviruses for Optically Guiding Electrophysiological Recordings and for Targeted Gene Delivery.- Selective Protein Expression Within the CNS Using Hybrid Lentivirus.- Construction and Analysis of Alphaherpesviruses Expressing Green Fluorescent Protein.- The Use of Green Fluorescent Fusion Proteins to Monitor Herpes Simplex Virus Replication.- Modified Bovine Herpesvirus 1 for Protein Secretion.- Identification of Viral Peptide Fragments for Vaccine Development.- Use of GFP to Investigate Expression of Plant-Derived Vaccines.- Studies of the Role and Function of Barley Stripe Mosaic Virus Encoded Proteins in Replication and Movement Using GFP Fusions.- Sendai Virus for Cancer Immunotherapy.- Poxvirus Tropism for Primary Human Leukocytes and Hematopoietic Cells.- Reverse Genetics of Nipah Virus to Probe Viral Pathogenicity.- Baculovirus-Mediated Gene Transfer into Mesenchymal Stem Cells.

Contains protocols useful for both basic researchers and for those interested in applied viral gene therapy Includes viral methods for terminally differentiated cells and for undifferentiated cells, including human stem cells Provides example applications for many different viral vectors including HIV, herpes virus, pox virus, cytomegalovirus, adenovirus and adeno-associated viruses, baculovirus, SARS, and many others Examines state-of-the-art imaging techniques including in vivo two-color fluorescence video micrsocopy and the combination of fluorescence imaging with PET, SPECT or MRI Describes viral applications applicable for Medicine, Neuroscience, Immunology, Agriculture and Laboratory Safety