Transmembrane β-Barrel Proteins, 1st ed. 2024 Methods and Protocols Methods in Molecular Biology Series, Vol. 2778

1. The Name is Barrel, β-Barrel

            Scout Hayashi, Susan K. Buchanan, and Istvan Botos

2. Recombinant Expression and Overproduction of Transmembrane b-Barrel Proteins

            Ina Meuskens, Jack C. Leo, and Dirk Linke

3. Bacterial Envelope Fractionation

            Athanasios Saragliadis and Dirk Linke

4. Fluorescent Labeling of Outer Membrane Proteins Using the SpyCatcher-SpyTag System

            Rachael Duodu, Dirk Linke, and Jack C. Leo

5. In Vitro Reconstruction of Bacterial β-Barrel Membrane Protein Assembly Using E. coli Microsomal (Mid-Density) Membrane

            Eriko Aoki, Edward Germany, and Takuya Shiota

6. Examining Protein Translocation by β-Barrel Membrane Proteins Using Reconstituted Proteoliposomes

            Minh Sang Huynh, Jiaming Caitlyn Xu, and Trevor F. Moraes

7. In Vivo Disulfide-Bond Crosslinking to Study β-Barrel Membrane Protein Interactions, Dynamicity, and Folding Intermediates

            Matthew Thomas Doyle

8. Site-Specific Photocrosslinking to Investigate Toxin Delivery Mediated by the Bacterial β-Barrel Assembly Machine

            Emily M. Bouzan and Christine L. Hagan

9. Analysis of Transmembrane β-Barrel Proteins by Native and Semi-Native Polyacrylamide Gel Electrophoresis

            Violette Morales, Luis Orenday-Tapia, and Raffaele Ieva

10. Affinity Purification of Membrane β-Barrel Proteins via Biotin-Tagged Peptidiscs

            Zhiyu Zhao, John William Young, and Franck Duong van Hoa

11. Monitoring the Interaction of the Peptidoglycan with the Bacterial β-Barrel Assembly Machinery

            Federico Corona and Waldemar Vollmer

12. MitoLuc: A Luminescence-Based Assay to Study Real-Time Protein Import into Mitochondria

            Holly C. Ford and Ian Collinson

13. Modular Assembly of Mitochondrial β-Barrel Proteins

            Rituparna Bhowmik, Fabian den Brave, and Thomas Becker

14. Tracking the Activity and Position of Mitochondrial β-Barrel Proteins

            Shuo Wang and Stephan Nussberger

15. Conformational Heterogeneity of β-Barrel Membrane Proteins Observed In Situ Using Orthogonal Spin Labels and Pulsed ESR Spectroscopy

            Sophie Ketter, Aathira Gopinath, and Benesh Joseph

16. Characterization of β-Barrel Outer Membrane Proteins and Their Interactions with Chaperones by Chemical-Crosslinking Mass Spectrometry

            Antonio N. Calabrese

17. Dissecting the Organization of a β-Barrel Assembly Machinery (BAM) Complex by Neutron Reflectometry

            Xiangfeng Lai and Hsin-Hui Shen

18. Expression, Purification, and Cryo-EM Structural Analysis of an Outer Membrane Secretin Channel

            Rebecca Conners, Mathew McLaren, Marjorie Russel, and Vicki A.M. Gold

19. Recent Advances in Modeling Membrane β-Barrel Proteins Using Molecular Dynamics Simulations: From Their Lipid Environments to Their Assemblies

            Anna L. Duncan, Ya Gao, Evert Haanappel, Wonpil Im, and Matthieu Chavent

20. Structural Modeling of T9SS Outer Membrane Proteins and Their Complexes

            Christian D. Lorenz, Michael A. Curtis, and James A. Garnett

21. Rationale in Custom Design of Transmembrane β-Barrel Pores

            Anastassia A. Vorobieva

22. Stress-Based Screening for Compounds that Inhibit β-Barrel Outer Membrane Protein Assembly in Gram-Negative Bacteria

            Laurence Cleenewerk, Joen Luirink, and Peter van Ulsen

Includes cutting-edge techniques

Provides step-by-step detail essential for reproducible results

Contains key implementation advice from the experts