Tissue-Resident Macrophages, 1st ed. 2024 Methods and Protocols Methods in Molecular Biology Series, Vol. 2713

1. Macrophage Development and Function

            Nikola Makdissi

2. Fate Mapping Macrophages: From Ontogeny to Functions

            Anna Ahlback and Rebecca Gentek

3. Studying Autophagy in Microglia: Overcoming the Obstacles

            Ainhoa Plaza-Zabala and Amanda Sierra

4. Hemocyte Nuclei Isolation from Adult Drosophila melanogaster for snRNA-Seq

            Fabian Hersperger, Melanie Kastl, Katrin Paeschke, and Katrin Kierdorf

5. Isolation of Tissue Macrophages in Adult Zebrafish

            Mireia Rovira, Jennifer Pozo, Magali Miserocchi, and Valérie Wittamer

6. Genetic and Immunohistochemistry Tools to Visualize Rat Macrophages In Situ

            Stephen Huang, Dylan Carter-Cusack, Emma Maxwell, Omkar L. Patkar, Katharine M. Irvine, and David A. Hume

7. Phenotyping of Macrophages in Human Immune System Mice

            Leonie Voss, Carmen Reitinger, and Anja Lux

8. Fate-Mapping of Yolk Sac-Derived Macrophages

            Iva Splichalova and Elvira Mass

9. Fate-Mapping of Hematopoietic Stem Cell-Derived Macrophages

            Katharina Mauel and Elvira Mass

10. Isolation and Flow Cytometry Analysis of Macrophages from White Adipose Tissue

            Dailia Ribeiro, Seniz Yüksel, Andreas Dolf, and Dagmar Wachten

11. Isolation and Flow Cytometry Analysis of Macrophages from the Dermis

            Aaron James Forde and Julia Kolter

12. Isolation and Flow Cytometry Analysis of Macrophages from the Kidney

            Sarah J. Bland, Alex Yashchenko, and Kurt A. Zimmerman

13. Isolation and Flow Cytometry Analysis of Intestinal Macrophages

            Maria Francesca Viola and Guy Boeckxstaens

14. Isolation and Characterization of Testis Macrophages Using Flow Cytometry

15. Studying Macrophages in the Murine Fatty Liver Using Flow Cytometry and Confocal Microscopy

            Zhuangzhuang Liu, Pieter A. Louwe, and Charlotte L. Scott

16. Isolation, Ex Vivo Expansion, and Lentiviral Transduction of Alveolar Macrophages

            Clara Jana-Lui Busch, Sethuraman Subramanian, Javier Linares, Jérémy Favret, Ridzky Anis Advent Yuda, and Michael H. Sieweke

17. Translatome Profiling of Tissue-Resident Macrophages Using the RiboTag Approach

            Jung-Seok Kim, Zhana Haimon, Sigalit Boura-Halfon, and Steffen Jung

18. Spectral Flow Cytometry Analysis of Resident Tissue Macrophages

            Wan Ting Kong, Mathilde Bied, and Florent Ginhoux

19. Unveiling Macrophage Heterogeneity and Their Spatial Distribution Using Multiplexed Tissue Imaging

            David Alejandro Bejarano and Andreas Schlitzer

20. 3D Imaging of Macrophages in Complete Organs

            Carole Siret and Serge A. van de Pavert

21. Whole-Mount Imaging of Adipose Tissue Macrophages

            Lydia Sorokin and Luis Henrique Corrêa

22. Functional In Vivo Imaging of Macrophages

            Anja Wegner, Ralph Palmisano, and Stefan Uderhardt

23. Elucidating Immune Monitoring of Tissue-Resident Macrophages by Intravital Microscopy

            Karolin W. Hublitz and Efstathios G. Stamatiades

24. Combined Host-Pathogen Fate Mapping to Investigate Lung Macrophages in Viral Infection

            Sebastian Baasch, Julia Henschel, and Philipp Henneke

25. Measuring the Metabolic State of Tissue-Resident Macrophages via SCENITH

            Andrea Vogel, Paulina Garcia Gonzalez, and Rafael J. Argüello

26. Analyzing Fcg-Receptor Interactions on Monocytes with the Proximity Ligation Assay (PLA)

            Sibel Kara and Falk Nimmerjahn

27. Studying Efferocytosis Dynamics in Tissue-Resident Macrophages Ex Vivo

            Irene Aranda-Pardos, Achmet Imam-Chasan, and Noelia Alonso-Gonzalez

28. Monitoring of Inflammasome Activation of Macrophages and Microglia In Vitro,Part 1:Cell Preparation and Inflammasome Stimulation

            Marta Lovotti, Matthew S.J. Mangan, Róisín M. McManus, Kateryna Shkarina, Matilde B. Vasconcelos, and Eicke Latz

29. Monitoring of Inflammasome Activation of Macrophages and Microglia In Vitro, Part 2:Assessing Inflammasome Activation

            Marta Lovotti, Matthew S.J. Mangan, Róisín M. McManus, Kateryna Shkarina, Matilde B. Vasconcelos, and Eicke Latz

30. Detection of G-Quadruplex DNA Structures in Macrophages

            Melanie Kastl, Fabian Hersperger, Katrin Kierdorf, and Katrin Paeschke

31. Adaptation of Human iPSC-Derived Macrophages Towards an Alveolar Macrophage-Like Phenotype Post Intra-Pulmonary Transfer into Murine Models

            Miriam Hetzel, Ingrid Gensch, Mania Ackermann, and Nico Lachmann

32. Tackling Tissue Macrophage Heterogeneity by SplitCre Transgenesis

            Sigalit Boura-Halfon, Rebecca Haffner-Krausz, Shifra Ben-Dor, Jung-Seok Kim, and Steffen Jung

33. Automated Cell Counting of Macrophages In Situ

            Ouzéna Bouadi and Tuan Leng Tay

34. Morphometric Analyses of Macrophages

            Jan N. Hansen

35. Combined Analysis of mRNA Expression and Open Chromatin in Microglia

            Rebekka Scholz, Desirée Brösamle, Xidi Yuan, Jonas J. Neher, and Marc Beyer

Includes cutting-edge techniques

Provides step-by-step detail essential for reproducible results

Contains key implementation advice from the experts