T-Cell Development, Softcover reprint of the original 1st ed. 2016 Methods and Protocols Methods in Molecular Biology Series, Vol. 1323

 Concise and easy-to-use, T-Cell Development: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Part I: Background Information

1. 200 Million Thymocytes and I: A Beginner’s Survival Guide to T cell Development

Melanie S. Vacchio, Thomas Ciucci and Rémy Bosselut

2. Development of gd T Cells, the Special Forces Soldiers of the Immune System

David L. Wiest

Part II: Core Approaches and Strategies

3. Genetic Tools to Study T Cell Development

Thomas Ciucci, Melanie S. Vacchio and Rémy Bosselut

4. Assessment of T cell Development by Flow Cytometry

Jan Y.M. Lee and Paul E. Love

5. Flow Cytometry Analysis of Thymic Cells and their Subpopulations

Izumi Ohigashi and Yousuke Takahama Epithelial

6. Identifying the Spatial Relationships of Thymic Stromal and Thymocyte Subsetsby Immunofluorescence Analysis

Virginia Bain and Ellen R. Richie

7. Purification of Thymocyte and T Cell Subsets

Andrea C. Carpenter, Jong Kyong Kim, and Rémy Bosselut

8. Retroviral Transduction of  T Cells and T Cell Precursors

Amie Simmons and José Alberola-Ila

9. Bone Marrow and Fetal Liver Radiation Chimeras

Francis A Flomerfeltand Ronald E Gress

10. In vitro Analyses of T Cell Effector Differentiation

Elizabeth A. Wohlfert,

Part III: Specific Techniques

11. Studying T Cell Development in Thymic Slices

Jenny O. Ross, Heather J. Melichar, Joanna Halkias, and Ellen A. Robey

12. FTOC-based Analysis of Negative Selection

Cody, A. Cunningham, Emma Teixeiro, and Mark A. Daniels

13. Reconstituted Thymus Organ Culture

Zimu Deng, Haifeng Liu, Jinxiu Rui^, and Xiaolong Liu

14. Induction of T-cell Development in vitro by Delta-like (Dll)-Expressing Stromal Cells

Mahmood Mohtashami, Payam Zarin and Juan Carlos Zúñiga-Pflücker

15. In vitro Analysis of Thymocyte Signaling

Cody A. Cunningham, Emma Teixeiro, Mark A. Daniels

16. Molecular Analysis of Mouse T Cell Receptor a and b Gene Rearrangements

Levi J. Rupp, Liang Chen, Michael S. Krangel, and Craig H. Bassing

17. Intrathymic Injection

Sugata Mannaand Avinash Bhandoola

18. Analysis of Cell Proliferation and |Homeostasis using EdU Labeling

Francis A Flomerfeltand Ronald E Gress

19.

Karin Davids, Inge Van de Walle and Tom Taghon

20. Approaches to Study Human T Cell Development

Anne-Catherine Dolens, Inge Van de Walle and Tom Taghon

21. Humanized Mice to Study Human T Cell Development

Sarah Bonte, Sylvia Snauwaert, Stijn Vanhee, Anne-Catherine Dolens, Tom Taghon, Bart Vandekerckhove, and Tessa Kerre

22. Using the Zebrafish Model to Study T cell Development

Yong Zhang and David L. Wiest

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts