Reverse Genetics of RNA Viruses (2^nd Ed., 2nd ed. 2024) Methods and Protocols Methods in Molecular Biology Series, Vol. 2733

This second edition volume expands on the previous edition with new and updated chapters that highlight the latest methods and approaches for the manipulation of RNA viruses.  The chapters in this book explore the fundamental role in studying RNA viruses; identifying markers of host range, disease, and transmission; and aid readers with the further development of in silico computational biology tools and artificial intelligence algorithms that can help predict the emergence of certain pathogens. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Reverse Genetics of RNA Viruses: Methods and Protocols, Second Edition is a valuable resource for researchers who are interested in learning more about this developing field.

Preface…

Table of Contents…

Contributing Authors… 

1. Reverse Genetics Systems for Filoviruses

Bianca S. Bodmer and Thomas Hoenen

2. Reverse Genetics Systems for the de novo Rescue of Diverse Members of Paramyxoviridae

Griffin Haas and Benhur Lee

3. Rescue of Recombinant Newcastle Disease Virus Expressing Heterologous Genes

Arantza Cobela-García, Ignacio Mena, and Adolfo García-Sastre

4. Use of Reverse Genetics for the Generation of Recombinant Influenza Viruses Carrying Nanoluciferase

C. Joaquin Caceras, L. Claire Gay, Flavio Cargnin Faccin, and Daniel R. Perez

5. Reverse Genetics of Bat Influenza A Viruses

Susanne Kessler, Adolfo Garcia-Sasre, Martin Schwemmle, and Kevin Ciminski

6. Rescue of Infectious Salmon Anemia Virus (ISAV) from Cloned cDNA

Daniela Toro-Ascuy, Matías Cárdenas, Yesseny Vásquez-Martínez, and Marcelo Cortez-San Martín

7. Reverse Genetics System for Rift Valley Fever Virus

Breanna Tercero and Shinji Makino

8. Plasmid-Based Lassa Virus Reverse Genetics

Luis Martínez-Sobrido, Chengjin Ye, and Juan Carlos de la Torre

9. Bacterial Artificial Chromosome Reverse Genetics Approaches for SARS-CoV-2

Kevin Chiem, Aitor Nogales, Fernando Almazan, Chengjin Ye, and Luis Martínez-Sobrido

10. Construction of Infectious Clones for Human Enteroviruses

Thinesshwary Yogarajah and Justin Jang Hann Chu

11. Reverse genetics of Hepatitis C Virus using an RNA Polymerase I-Mediated Transcription

Ryosuke Suzuki and Tetsuro Suzuki

12. Reverse Genetics of Zika Virus using a Bacterial Artificial Chromosome

Aitor Nogales, Luis Martínez-Sobrido, and Fernando Almazán

Zhong-Yu Liu, Xiao-Feng Li, and Cheng-Feng Qin

14. Reverse Genetics of Dengue Virus

José Valter Joaquim Silva Junior, Andréa Nazaré Monteiro Rangel da Silva, Jefferson José da Silva Santos, and Laura Helena Vega Gonzales Gil

Chantal A. Agbemabiese, Asha A. Philip, and John T. Patton

Subject Index List…