Pyroptosis, 2023 Methods and Protocols Methods in Molecular Biology Series, Vol. 2641

Molecular Mechanisms of Pyroptosis.- Assessment of NLRP3 Inflammasome Activation and NLRP3-NEK7 Complex Assembly.- Inducing Pyroptosis with FlaTox, RodTox, or NeedleTox.- Inducing Pyroptosis via the Pyrin Inflammasome.- Caspase-4 Activation and Recruitment to Intracellular Gram-Negative Bacteria.- CARD8 Inflammasome Activation by HIV-1 Protease.- Pyroptosis Markers in Human Primary Specimens: Quantification of Intracellular ASC Specks by Imaging Flow Cytometry and Extracellular Oxidized Mitochondrial by ELISA.- Identifying Inflammasome Activation in Human Tissues.- Analyzing Caspase-8-Dependent GSDMD Cleavage in Response to Yersinia Infection.- Biochemical Methods for Assessing Gasdermin D Inactivation in Macrophages.- Detection of Gasdermin C-Mediated Cancer Cell Pyroptosis.- Inducing Pyroptosis via Gasdermin B and Gasdermin E Cleavage.- Assessing Interleukin-1β Activation during Pyroptosis.- Monitoring Calcium Fluxes and Lysosome Exocytosis during Pyroptosis.- Assessing Cytoskeletal Destruction During Pyroptosis.- Assessment of HMGB1 Release During Experimentally-Induced Pyroptosis.- Time-Lapse Imaging of Inflammasome-Dependent Cell Death and Extrusion in Enteroid-Derived Intestinal Epithelial Monolayers.