Potassium Channels, 1st ed. 2024 Methods and Protocols Methods in Molecular Biology Series, Vol. 2796

Preface…
Table of Contents…
Contributing Authors…

1. Asymmetric Lipid Bilayers and Potassium Channels Embedded therein in the Contact Bubble Bilayer
Yuka Matsuki, Masayuki Iwamoto, and Shigetoshi Oiki

2. Detecting Bound Ions in Ion Channels by Solid-State NMR Experiments on 15N Labelled Ammonium Ions
Carl Öster, Sascha Lange, Kitty Hendriks, and Adam Lange

3. Conformational Dynamic Studies of Prokaryotic Potassium Channels Explored by Homo-FRET Methodologies
Ana Coutinho, José Antonio Poveda, and María Lourdes Renart

4. Purification of Potassium Ion Channels Using Styrene-Maleic Acid Copolymers
Grigory Glukhov, Maria Karlova, Ekaterina Kravchuk, Anna Glukhova, Elizaveta Trifonova, and Olga S. Sokolova

5. Ready to Record Cells for Kinetic Screening of VGICs
Emmanuelle Logette and Rajnish Ranjan

6. Cell-Based Thallium-Influx Fluorescence Assay for Kv10.1 Channels
María Luisa Durán-Pastén and Enoch Luis

7. Use of Surface Plasmon Resonance Technique for Studies of Inter-Domain Interactions in Ion Channels
Purushottam B. Tiwari, Pareesa Kamgar-Dayhoff, Prakriti Tiwari, Maria I. McKillop, and Tinatin I. Brelidze

8. Voltage Clamp Fluorometry: Illuminating the Dynamics of Ion Channels
Daniel Sastre and David Fedida

9. Creating Computational Models of Ion Channel Dynamics
Max E. Schoening and Jonathan R. Silva

10. Human Kir2.1 Potassium Channel: Protocols for Cryo-EM Data Processing and Molecular Dynamics Simulations
Carlos A. H. Fernandes and Catherine Vénien-Bryan

11. Evaluation of the Role of Potassium Channels in the Proliferation, Migration, and Invasion of Blood Cells
Valeria Y. Vasileva and Vladislav I. Chubinskiy-Nadezhdin

12. Non-Radioactive Rb+ Efflux Assay for Screening KATP Channel Modulators
Assmaa ElSheikh, Camden M. Driggers, and Show-Ling Shyng

13. The Dynamic Clamp Technique: A Robust Toolkit for Investigating Potassium Channel Function
Chiara Bartolucci and Luca Sala

14. Automated Patch Clamp Recordings of GPCR-Gated Ion Channels: Targeting the MC4-R/Kir7.1 Potassium Channel Complex
Ciria C. Hernandez, Luis E. Gimenez, and Roger D. Cone

15. Machine Learning Methods for the Analysis of the Patch-Clamp Signals
Monika Richter–Laskowska, Agata Wawrzkiewicz-Jalowiecka, Aleksander Bies, and Paulina Trybek

16. Isothermal Titration Calorimetry for Fragment-Based Analysis of Ion Channel Interactions
Crystal Archer

Subject Index List…

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts