Permeability Barrier (2^nd Ed., 2nd ed. 2021) Methods and Protocols Methods in Molecular Biology Series, Vol. 2367

1. Use of Ussing Chambers to Measure Paracellular Permeability to Macromolecules in Mouse Intestine

            Doriane Aguanno, Barbara Graziela Postal, Véronique Carrière, and Sophie Thenet

2. Rapid Evaluation of Intestinal Paracellular Permeability Using the Human Enterocytic-Like Caco-2/TC7 Cell Line

            Barbara Graziela Postal, Doriane Aguanno, Sophie Thenet, and Véronique Carrière

3. Evaluation of Barrier Functions in Human iPSC-Derived Intestinal Epithelium

            Shigeru Yamada and Yasunari Kanda

4. Selective Regional Isolation of Brain Microvessels

            Fernanda Medina-Flores, Gabriela Hurtado-Alvarado, and Beatriz Gómez-González

5. Estimating Brain Permeability Using In Vitro Blood-Brain Barrier Models

            Saeideh Nozohouri, Behnam Noorani, Abraham Al-Ahmad, and Thomas J. Abbruscato

6. In Vitro Human Blood-Brain Barrier Model for Drug Permeability Testing

            Ece Bayir and Aylin Sendemir

7. Evaluation of Blood-Brain Barrier Integrity Using Vascular Permeability Markers: Evans Blue, Sodium Fluorescein, Albumin-Alexa Fluor Conjugates, and Horseradish Peroxidase

            Bulent Ahishali and Mehmet Kaya

8. Blood-Brain Barrier (BBB) Permeability and Transport Measurement In Vitro and In Vivo

9. Assessment of Blood-Brain Barrier Permeability Using Miniaturized Fluorescence Microscopy in Freely-Moving Rats

            Jeffrey L. Barr, G. Cristina Brailoiu, Ellen M. Unterwald, and Eugen Brailoiu

10. Measurement of Lung Vessel and Epithelial Permeability In Vivo with Evans Blue

            Prestina Smith, Lauren A. Jeffers, and Michael Koval

11. Measurement of Airway Epithelial Permeability: Methods and Protocols

12. Vascular Permeability Assays In Vivo

            Mir S. Adil and Payaningal R. Somanath

13. Endothelial Permeability Assays In Vitro

            Mir S. Adil and Payaningal R. Somanath

14. Mapping Receptor Antibody Endocytosis and Trafficking in Brain Endothelial Cells

            Mikkel R. Holst, Simone S.E. Nielsen, and Morten S. Nielsen

15. An In Vitro Assay to Monitor Sertoli Cell Blood-Testis Barrier (BTB) Integrity

            Siwen Wu, Lingling Wang, Elizabeth I. Tang, Junlu Wang, and C. Yan Cheng

16. Ussing Chamber Methods to Study the Esophageal Epithelial Barrier

            Solange M. Abdulnour-Nakhoul and Nazih L. Nakhoul

17. A Simple Method to Test Mechanical Strain on Epithelial Cell Monolayers Using a 3D-Printed Stretcher

            Amanda C. Daulagala, John Yost, Amirreza Yeganegi, William J. Richardson, Michael J. Yost, and Antonis Kourtidis

18. Differentiating between Tight Junction-Dependent and Tight Junction-Independent Intestinal Barrier Loss In Vivo

            Sandra D. Chanez-Paredes, Shabnam Abtahi, Wei-Ting Kuo, and Jerrold R. Turner

19. Establishment of Intestinal Epithelial Cell Monolayers and Their Use in Calcium Switch Assay for Assessment of Intestinal Tight Junction Assembly

            Pawin Pongkorpsakol, Wilasinee Satianrapapong, Preedajit Wongkrasant, Peter R. Steinhagen, Nuttha Tuangkijkul, Nutthapoom Pathomthongtaweechai, and Chatchai Muanprasat

20. A Method to Prepare a Bioprobe for Regulatory Science of the Drug Delivery System to the Brain: An Angulin Binder to Modulate Tricellular Tight Junction-Seal

            Keisuke Tachibana and Masuo Kondoh

21. dSTORM Imaging and Analysis of Desmosome Architecture

            Reena R. Beggs, William F. Dean, and Alexa L. Mattheyses

Includes cutting-edge techniques

Provides step-by-step detail essential for reproducible results

Contains key implementation advice from the experts