Microbial Environmental Genomics (MEG) (2^nd Ed., 2nd ed. 2023) Methods in Molecular Biology Series, Vol. 2605

Metabarcoding Approaches For Soil Eukaryotes, Protists and Microfauna.- Metabarcoding of the three domains of life in aquatic saline ecosystems.- Sample preparation for fungal community analysis by high-throughput sequencing of barcode amplicons.- Characterization of seed mycobiota using culture-dependent and -independent approaches.- Tissue Cultivation, Preparation, and Extraction of High Molecular Weight DNA for Single-Molecule Genome Sequencing of Plant-Associated Fungi.- Analysis of ancient microbial DNA.- Whole Genome Sequencing of bacterial endophytes from fresh and preserved plant specimens.- Investigating the bacterial and fungal communities involved in dead biomass degradation in forest soils.- Stable isotope probing-RNA strategy to study plant/fungus interactions.- Targeted 16S rRNA Gene Capture by Hybridization and Bioinformatic Analysis.- Construction of a transposon mutant library in the pathogen Agrobacterium tumefaciens C58 and identification of genes involved in gall niche exploitation and colonization.- High-throughput screening of fosmid libraries for increased identification of novel N-acyl homoserine lactone degrading enzymes.- Analyzing prokaryotic transcriptomics in the light of genome data with the MicroScope platform.- MycoCosm, the JGI’s Fungal Genome Portal for Comparative Genomic and Multiomics Data Analyses.- Investigating endobacteria that thrive within Mucoromycota.- Confocal laser scanning microscopy approach to investigate plant-fungal interactions.- Visualisation of fungi during wood colonisation and decomposition by microscopy: from light to electron microscopy.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts