In Vitro Methods in Toxicology

First published in 1987, this book describes tissue (cell and organ) culture techniques which could be used at a critical predevelopment stage to screen for drug-inducing toxicity or to analyse the molecular mechanisms of toxicity at a later stage in parallel with investigative studies. This type of research has important ethical, financial and scientific implications. In particular, less animals and compound are required in in vitro research and more detailed information at the cellular and subcellular levels can be obtained. The volume brings together the once disparate knowledge of cultural techniques and thus provides an important reference work for industrial and academic toxicologists. It could also form a framework for discussion of potential improvements and modification of toxicity testing.

Introduction G. B. Leslie and P. Johnson; Part I. Target Organ Toxicity: 1. In vitro methods in renal toxicology M. A. Snith, W. R. Hewitt and J. Hook; 2. Ototoxicity E. S. Harpur; 3. Cultured cell models for studying problems in cardiac toxicology N. Sperelakis and G. Bkaily; 4. Use of the isolated rat heart for the detection of cardiotoxic agents A. Cockburn; 5. Brain reaggregate cultures in neurotoxicological investigations C. K. Atterwill; 6. Application of thyroid cell culture to the study of thyrotoxicity C. G. Brown; 7. In vitro methods to investigate toxic lung disease A. Poole and R. C. Brown; 8. Metabolism and toxicity of drugs in mammalian hepatocyte culture R. J. Chenery; 9. In vitro evaluation of haemic systems in toxicology J. S. H. Luke and G. R. Betton; Part II. General and Topical Toxicity: 10. The chorioallantoic membrance in irritancy testing R. S. Lawrence; 11. In vitro dermal irritancy tests P. A. Duffy and O. P. Flint; 12. Genetic toxicology M. Richold; 13. In vitro immunotoxicology K. Miller, S. Nicklin and S. G. Volsen; Part III. Reproductive Toxicity: 14. An in vitro test for teratogens using cultures of rat embryo cells O. P. Flint; 15. Postimplantion embryo culture and its application to problems in teratology D. L. Cockroft and C. E. Steele; 16. Sub-mammalian and sub-vertebrate models in teratogenicity screening S. J. Freeman and N. A. Brown; 17. The use of in vitro techniques to investigate the action of testicular toxicants D. A. Garside; 18. An in vitro assessment of ovarian function: a potential tool for investigating mechanisms of toxicity in the ovary D. A. Garside and P. McKibbin; Part IV. Conclusion: 19. Conclusion C. K. Atterwill and C. E. Steele; Index.