Imaging Cell Signaling, 1st ed. 2024 Methods in Molecular Biology Series, Vol. 2800

This volume explores recent innovations across the entire pipeline of imaging signal transduction, cell preparation, cellular manipulation, image acquisition, and computational analysis. The chapters in this book cover topics such as rapid preparation of living Drosophila pupal macrophages for ex vivo imaging; controlling the potency of T cell activation using an optically tuneable chimeric antigen receptor; in situ imaging of proteins using DNA-PAINT super-resolution microscopy; reconstructing signaling networks using biosensor barcoding; and morphological, spatial, and dynamic models for cellular components. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and authoritative, Imaging Cell Signaling is a valuable resource that provides guidance to researchers looking to learn how to effectively tailor design projects to image, manipulate, and model signal transduction.

Preface…
Table of Contents…
Contributing Authors…

1. Rapid Preparation of Living Drosophila Pupal Macrophages for Ex Vivo Imaging
Tua Karling and Helen Weavers

2. Generation of 3D Fibroblast-Derived Extracellular Matrix and Analysis of Tumor Cell-Matrix Interactions and Signalling
Emily Lay, Tressan Grant, Gernot Walko, and Ute Jungwirth

3. Induced Formation of Plasma Membrane Protrusions with Porous Materials as Instructive Surfaces
Morteza Aramesh and Cecilia Persson

4. Design and Synthesis of DNA Origami Nanostructures to Control TNF Receptor Activation 
Göktuğ Aba, Ferenc A. Scheeren, and Thomas H. Sharp

5. Controlling the Potency of T Cell Activation using an Optically Tuneable Chimeric Antigen Receptor
Muna Fuyal and John R. James

6. Imaging Cell Signaling in Tissues using the IBEX Method
Yuxuan Meng, Changtai Li, Gregory S. Patient, Yuan Tian, and Colin J. Chu

7. Investigating Epidermal Growth Factor Receptor Trafficking with High-Resolution Enzymatic Protein-Tagging and Transmission Electron Microscopy 
Nicholas Ariotti

8. Some Guiding Principles for a "Simple" Correlative Light Electron Microscopy Experiment 
Elina Mäntylä and Paul Verkade

9. In Situ Imaging of Proteins using DNA-PAINT Super-Resolution Microscopy
Florian Schueder and Ralf Jungmann

10. A Magnetic Pincher for the Dynamic Measurement of the Actin Cortex Thickness in Live Cells
Joseph Vermeil, Valentin Laplaud, Anumita Jawahar, Dulamkhuu Bujaa, Damien Cuvelier, Julien Heuvingh, Olivia du Roure, and Matthieu Piel

11. Measurement of Forces Acting on Single T Cell Receptors
Lukas Schrangl, Janett Göhring, Florian Kellner, Johannes B. Huppa, and Gerhard J. Schütz

12. A Pipeline for Dynamic Analysis of Mitochondrial Content in Developing T Cells: Bridging the Gap between High Throughput Flow Cytometry and Single-Cell Microscopy Analysis
Vaibhav Dhyani, Anchi S. Chann, Lopamudra Giri, Sarah M. Russell, and Mirren Charnley

13. Reconstructing Signaling Networks using Biosensor Barcoding
Suyang Wang, Wei-Yu Chi, Gabriel Au, Cheng-Chieh Huang, Jr-Ming Yang, and Chuan-Hsiang Huang

14. Deep Learning-Based Cell Tracking in Deforming Organs and Moving Animals
Chentao Wen

15. Using Dimensionality Reduction to Visualize Phenotypic Changes in High-Throughput Microscopy
Alex X. Lu and Alan M. Moses

16. Learning Morphological, Spatial, and Dynamic Models for Cellular Components
Huangqingbo Sun and Robert F. Murphy

Subject Index List…

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts