Host-Pathogen Interactions (2^nd Ed., 2nd ed. 2024) Methods and Protocols Methods in Molecular Biology Series, Vol. 2751

Part I: Introduction

1. Host-Pathogen Interaction: Biology and Public Health

            Richard Ponce-Cusi, Leny Bravo, Kevin J. Paez, Joseph A. Pinto, and Nesstor Pilco-Ferreto

2. Genetic Association Studies in Host-Pathogen Interaction Analysis

            Marina Laplana, José Luis Royo, and Luis Miguel Real

Part II: Viral-Host Interactions

3. Live-Cell Fluorescence Imaging for Virus/Host Interactions

            Francesca J. Scribano, Kristen A. Engevik, J. Thomas Gebert, and Joseph M. Hyser

4. Construction of a Mycoviral Infectious Clone for Reverse Genetics in Botrytis cinerea

            Laura Córdoba, Ana Ruiz-Padilla, Javier Pardo-Medina, Julio L. Rodríguez-Romero, and María A. Ayllón

Part III: Plant-Pathogens Interactions

5.  Exopolysaccharide Production and Precipitation Method as a Tool to Study Virulence Factors

            Natalia Mielnichuk, Constanza M. Joya, María A. Monachesi, and Romina P. Bertani

6. Virulence-Related Assays for Investigation of the Acidovorax citrulli-Cucurbitaceae Pathosystem

            Francisco Pérez-Montaño, Irene Jiménez-Guerrero, Dafna Tamir-Ariel, and Saul Burdman

7. Dual-Fluorescence Chromosome-Located Labeling System for Accurate In Vivo Single-Cell Gene Expression Analysis in Pseudomonas syringae

            Nieves López-Pagán, José S. Rufián, Javier Ruiz-Albert, and Carmen R. Beuzón

8. A Robust Method to PerformIn Vitro and In Planta Interbacterial Competition Assays: Killing Plant Pathogens by a Potent Biocontrol Agent

            Cristina Civantos, Adrián Ruiz, and Patricia Bernal

Part IV: Molecular Methods for Plant-Interacting Bacteria

9. Quantification of Mixed-Linkage β-Glucan (MLG) in Bacteria

            Juan Antonio Marchante, Lucía Ruíz-Sáez, Socorro Muñoz, Juan Sanjuán, and Daniel Pérez-Mendoza

10. Surface Plasmon Resonance as a Tool to Elucidate the Molecular Determinants of Key Transcriptional Regulators Controlling Rhizobial Lifestyles

            Laura Tomás-Gallardo, Juan J. Cabrera, and Socorro Mesa

11. Microscope Subcellular Localization of Plant-Interacting Bacterial Effectors in Animal Cell Cultures

            Irene Jiménez-Guerrero, Francisco Javier López-Baena, and Carlos Medina

12. A Workflow for the Functional Characterization of Noncoding RNAs in Legume Symbiotic Bacteria

13. Methods for Studying Swimming and Surface Motilities in Rhizobia

            Francisco Fuentes-Romero, Cynthia Alías-Villegas, Pilar Navarro-Gómez, Sebastián Acosta-Jurado, Lydia M. Bernabéu-Roda, Virginia Cuéllar, María J. Soto, and José M. Vinardell

14. Isolation, Quantification, and Visualization of Extracellular Membrane Vesicles in Rhizobia Under Free-Living Conditions

            Paula Ayala-García, Natalia Moreno-de Castro, Irene Jiménez-Guerrero, Mathias Müsken, Alejandro Arce-Rodríguez, Francisco Pérez-Montaño, and José Manuel Borrero-de Acuña

15. Isolation of Rhizobial Extracellular Membrane Vesicles from Bacteroids

            Paula Ayala-García, Irene Jiménez-Guerrero, Mathias Müsken, Francisco Javier Ollero, José Manuel Borrero-De Acuña, and Francisco Pérez-Montaño

16. Nod Factor Lipopolysaccharide Purification to Study Nitrogen-Fixing Bacteria Symbiosis with Legumes

            Catherine Jacott, Sara Lozano-Morillo, and Pablo del Cerro

17. A Novel System to Selective Tagging of Sinorhizobium fredii Symbiotic Plasmids

            Ana María Cutiño, María del Carmen Sánchez-Aguilar, José Enrique Ruiz-Sáinz, María del Rosario Espuny, Francisco Javier Ollero, and Carlos Medina

18. New Inoculation Strategy for Legume Based on Rhizobium-Metabolite Co-Encapsulation

            Adriana B. Cesari, Verónica E. Castilla Marín, Luciana Nieva Muratore, Natalia S. Paulucci, and Marta S. Dardanelli

Includes cutting-edge techniques

Provides step-by-step detail essential for reproducible results

Contains key implementation advice from the experts