Hepatic Stellate Cells, 1st ed. 2023 Methods and Protocols Methods in Molecular Biology Series, Vol. 2669
Coordonnateurs : Weiskirchen Ralf, Friedman Scott L.
1. Isolation, Purification, and Culture of Primary Murine Hepatic Stellate Cells: An Update
Steffen K. Meurer, Sabine Weiskirchen, Carmen G. Tag, and Ralf Weiskirchen
2. Differentiation of Hepatic Stellate Cells from Pluripotent Stem Cells
Raquel A. Martínez García de la Torre and Pau Sancho-Bru
3. Testing Cell Migration, Invasion, Proliferation, and Apoptosis in Hepatic Stellate Cells
Miriam Wankell and Lionel Hebbard
4. Phalloidin Staining for F-Actin in Hepatic Stellate Cells
Sarah K. Schröder, Carmen G. Tag, Sabine Weiskirchen, and Ralf Weiskirchen
5. Retinyl Ester Analysis by Orbitrap Mass Spectrometry
Jeroen W.A. Jansen, Maya W. Haaker, Esther A. Zaal, and J. Bernd Helms
6. Studying Hepatic Stellate Cell Senescence
Sandra A. Serna-Salas, Abel A. Soto-Gámez, Zongmei Wu, Myrthe Klaver, and Han Moshage
7. Isolation of Hepatic Stellate Cells and Lymphocytes for Co-Culture Systems
Hee-Hoon Kim, Kyurae Kim, Song Hwa Hong, and Won-Il Jeong
8. Working with Immortalized Hepatic Stellate Cell Lines
Scott Friedman and Ralf Weiskirchen
9. Induction of Obstructive Cholestasis in Mice
Ralf Weiskirchen, Sabine Weiskirchen, Carmen G. Tag, and Steffen K. Meurer
10. Mouse Models for Hepatic Stellate Cell Activation and Liver Fibrosis Initiation
Yulia A. Nevzorova, Ralf Weiskirchen, and Christian Liedtke
11. Generation and Culture of Primary Mouse Hepatocyte-HSC Spheroids
Inge Mannaerts, Nathalie Eysackers, and Leo A. van Grunsven
12. Hepatic Stellate Cell Depletion and Genetic Manipulation
Qiuyan Sun and Robert F. Schwabe
13. Human Hepatic Stellate Cells: Isolation and Characterization
Xiao Liu, David A. Brenner, and Tatiana Kisseleva
14. Decellularization of Human Liver to Generate Native Extracellular Matrix for Use in Automated Functional Assays with Stellate Cells
Emma L. Shepherd, Ellie Northall, Pantelitsa Papakyriacou, Karolina Safranska, Karen Sorensen, and Patricia F. Lalor
15. Multiplex Immunostaining to Spatially Resolve the Cellular Landscape in Human and Mouse Livers
Adrien Guillot, Marlene Sophia Kohlhepp, and Frank Tacke
16. Single Cell Secretome Analyses of Hepatic Stellate Cells: Aiming for Single Cell Phenomics
Richell Booijink, Leon Terstappen, and Ruchi Bansal
17. Hepatic Stellate Cells Targeting Using Peptide-Modified Biologicals
Ruchi Bansal and Klaas Poelstra
18. Experimental Workflow for Preclinical Studies of Human Antifibrotic Therapies
Lien Reolizo, Michitaka Matsuda, and Ekihiro SekiIncludes cutting-edge techniques
Provides step-by-step detail essential for reproducible results
Contains key implementation advice from the experts
Date de parution : 05-2023
Ouvrage de 309 p.
17.8x25.4 cm
