Gene biotechnology, (2^nd Ed.)

Covering state-of-the-art technologies and a broad range of practical applications, the second edition of Gene Biotechnology provides coverage that both researchers and students need to understand and apply today's biotechnology techniques. It discusses a wide variety of approaches, from very basic methods to the latest, most sophisticated technologies, including gene manipulation methods. The book contains clearly detailed, step-by-step explanations of protocols with helpful troubleshooting tips. It addresses the needs of researchers in institutional and commercial environments, serves as a guide for graduate students designing, implementing, and evaluating experimental projects.

Approaches to Novel Research. Rapid Isolation of Specific cDNAs or Genes by PCR New! high throughput mrna profiling via cdna microarrays or dna chipsNew! mRNA Profiling by Quantitative RT-PCR Technique New! Functional Genome vs. Drug Discovery Construction and Screening of Subtracted and Complete Expression cDNA Libraries Subcloning of Gene or cDNAs Nonisotopic and isotopic dna or rna sequencingInformation Superhighway and Computer Databases of Nucleic Acids and Proteins Characterization of DNA or Genes by Southern Blot Hybridization Gene Overexpression by Sense RNA in Mammalian Systems Analysis of gene expression at the rna levelAnalysis of Gene Expression at the Protein Level New! Proteomics vs. Drug Discovery New! Phage Display Technology DNA or mRNA Analysis by Radioactive in situ Hybridization RT-PCR Detection of Low Abundance mRNAs Isolation and characterization of genes from genomic dna librariesCulture of Mouse Embryonic Stem Cells as a Model Mammalian Cell Line for Gene Expression Studies New Strategies for Gene Knockout Generation of Random Chimeras for Functional Assays of Genes Rapid generation of mutant dna constructs by site-specific mutagenesisLarge-scale Expression and Purification of Recombinant Proteins