Flow cytometry for research scientists : principles and applications

An essential handbook for research scientists that use, or plan to use, flow cytometers. Provides an introduction and guide for those new to the field while serving as a valuable reference for experienced users who want to investigate new applications. Aimed especially at the research scientist wishing to explore the applications of flow cytometry in a wide range of scientific fields, this book covers basic principles, instrumentation, data analysis, molecular cytometry, DNA analysis, and many practical applications. Fully illustrated throughout with a comprehensive index and useful appendices.

1. Introduction to the Field of Cytometry and its Importance in Biomedicine. 2. Overview of Flow Cytometry Instruments. 3. Flow Cytometric Data Analysis. CellQuest Software. 4. DNA Analysis. DNA Measurement and Cell Cycle Analysis. 5. Molecular Cytometry . 6. Surface Staining and Immunophenotyping Using Multicolor Analysis. 7. Handling of Samples: Biosafety. 8. Intracellular Antigens. Cytokines and the Study of Viral Antigens. 9. Flow Cytometric Assessment of Sperm Quality and Cell Cycle Analysis. 10. Flow Cytometric Assessment of Allopurinol Susceptibility in Leishmania infantum Promastigote. 11. Flow Cytometric Assessment of Transduction Efficiency and Vector Cytotoxicity of HSV-1 Amplicon Vectors. 12. Immunophenotyping of DC by Flow Cytometry and Description of Diverse Functional Studies. 13. Summary. 14. Protocols.

lI>Flow Cytometry for Research Scientistsl/I> provides an introduction and guide for those new to the field while serving as a valuable reference for experienced users who want to investigate new application.