Ferroptosis, 1st ed. 2023 Methods and Protocols Methods in Molecular Biology Series, Vol. 2712

This volume provides a comprehensive collection of experimental protocols for investigating ferroptosis in different systems, including cultured cells, animal models, and human tissues. The techniques covered in this book look at various aspects of ferroptosis ranging from the detection of lipid peroxidation to the measurement of glutathione peroxidase activity and the evaluation of mitochondrial morphology. Chapters also discuss basic molecular biology methods such as quantitative PCR and immunoblotting, and advanced imaging techniques such as transmission electron microscopy and confocal fluorescence microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and authoritative, Ferroptosis: Methods and Protocols is a valuable resource for researchers who are interested in studying ferroptosis in different contexts including basic research, drug discovery, and clinical applications.

Preface…
Table of Contents…
Contributing Authors…

1. Fluorogenic Probes for Intracellular Iron Detection
Runliu Wu, Daolin Tang, and Rui Kang

2. Stratifying Ferroptosis Sensitivity in Cells and Tissues with PALP
Fengxiang Wang, Nathchar Naowarojna, and Yilong Zou

3. ChIP and ChIRP Assays in Ferroptosis
Zuli Wang, Tania Tao, and Yongguang Tao

4. PAR-CLIP Assay in Ferroptosis
Xiangfei Xue, Manyuan Wang, Xiao Zhang, Lifang Ma, and Jiayi Wang

5. Organoids Models of Pancreatic Duct Adenocarcinoma
Chunhua Yu, Rui Kang, and Daolin Tang

6. Probing Lipid Peroxidation in Ferroptosis: Emphasizing the Utilization of C11-BODIPY-Based Protocols
Zhangshuai Dai, Wanting Zhang, Zhendong Yuan, and Junqi Huang

7. Membrane Integrity Assay in Ferroptosis
Chao Deng and Yangchun Xie

8. LC-MS-Based Redox Phosphoipidomics Analysis in Ferroptosis
Wan-Yang Sun, Rong Wang, and Rong-Rong He

9. Monitoring Lysosome Function in Ferroptosis
Fangquan Chen, Rui Kang, Daolin Tang, and Jiao Liu

10. Monitoring Mitochondria Function in Ferroptosis
Fangquan Chen, Jiao Liu, Daolin Tang, and Rui Kang

11. Generation of Organoids and Analysis of Ferroptosis in Organoids
Wenxin Li, Yujie Su, Jingyi Guo, Mengfei Wang, and Xingguo Liu

12. Analysis of Protein Degradation in Ferroptosis
Zhuojun Zhang and Lili Jiang

13. Lipidomics Analysis in Ferroptosis
Lin Zhi and Minghua Yang

14. In-Cell Western Assay in Ferroptosis
Jiayi Wang and Jingbo Li

15. Flow Cytometric Analysis of Regulated Cell Death
Siyuan Huang and Ling Zeng

16. Thermal Shift Assay in Ferroptosis
Sridhar Bammidi, Stacey Hose, James T. Handa, Debasish Sinha, and Sayan Ghosh

17. Detection of Ferroptosis in Patient-Derived Tumor Models
Wenjing Zhang and Yang Wang

18. Assessment of Ferroptosis in Hematopoietic Stem and Progenitor Cells
Yifan Zhang, Zhiyang Chen, Zhenyu Ju, and Qian Hu

19. Detection of Ferroptosis by Immunohistochemistry and Immunofluorescence
Xiao Zhong and Ruochan Chen

20. Analysis of MicroRNAs in Ferroptosis
John G. Yuen, Ga-Ram Hwang, and Jingfang Ju

21. Detection of Ferroptosis in Models of Brain Diseases
Danmin Shen, Fei Yang, and Qian Li

Subject Index List…

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts