Extracellular Vesicles in Diagnosis and Therapy, 1st ed. 2022 Methods in Molecular Biology Series, Vol. 2504

Part I: Extracellular Vesicles Detection and Isolation

1. Aptasensors for Cancerous Exosome Detection

            Jin Li, Sitao Xie, Fengli Qu, and Weihong Tan

2. Detection of Cancer-Derived Exosomes Using a Sensitive Colorimetric Aptasensor

            Lizhou Xu and Khuloud T. Al-Jamal

3. Isolation of Circulating Extracellular Vesicles by High-Performance Size-Exclusion Chromatography

            Kaloyan Takov, I-Jou Teng, and Manuel Mayr

4. Isolation and Proteomic Analysis of Mouse Serum Small Extracellular Vesicles for Individual Subject Analysis

            Federica Anastasi, Marialaura Dilillo, Davide Pellegrini, and Liam A. McDonnell

5. Protocol for Measuring Concentrations of Extracellular Vesicles in Human Blood Plasma with Flow Cytometry

            Najat Hajji, Chi M. Hau, Rienk Nieuwland, and Edwin van der Pol

Part II: Isolation and Characterization of Tissue and Biofluid-Specific EVs

6. Targeted Mass Spectrometry-Based Proteomics Method to Quantify Placental Extracellular Vesicles

            Andrew Lai, Carlos Palma, Alexis Salas, Flavio Carrion, and Carlos Salomon

7. Isolation and Characterization of Extracellular Vesicles Secreted from Human Pluripotent Stem Cell-Derived Cardiovascular Progenitor Cells

            Qiang Wu, Min-Xia Ke, and Huang-Tian Yang

8. Isolation and Characterization of Salivary Exosomes for Cancer Biomarker Discovery

            Lucas Trevisan França de Lima, Juliana Müller Bark, Mohammad Rasheduzzaman, Chameera Ekanayake Weeramange, and Chamindie Punyadeera

9. Isolation and Characterization of Urinary Extracellular Vesicles for MicroRNA Biomarker Signature Development with Reference to MISEV Compliance

            Marlene Reithmair, Anja Lindemann, Veronika Mussack, and Michael W. Pfaffl

Part III: Analysis of EV Components

10. Direct Detection of Extracellular Vesicle miRNAs Using a Single-Step RT-qPCR Assay

            Ayyanar Sivanantham, Heedoo Lee, and Yang Jin

11. Purification and Phosphoproteomic Analysis of Plasma-Derived Extracellular Vesicles

            Anton B. Iliuk

12. Lipidomic Analysis of Extracellular Vesicles Isolated from Human Plasma and Serum

            Yuchen Sun, Kosuke Saito, and Yoshiro Saito

Part IV: EV Engineering

13. Extracellular Vesicles and their Use as Vehicles of Immunogens

            Chiara Chiozzini, Barbara Ridolfi, and Maurizio Federico

14. Isolation and Fluorescent Labeling of Extracellular Vesicles from Cultured Tumor Cells

            Noelle Leary, Sarina Walser, and Lothar C. Dieterich

15. Generation, Characterization, and Count of Fluorescent Extracellular Vesicles

            Flavia Ferrantelli, Valentina Tirelli, Valeria Barreca, and Francesco Manfredi

16. Optimized Protocol for Plasma-Derived Extracellular Vesicles Loading with Synthetic miRNA Mimic Using Electroporation

            Margherita A.C. Pomatto, Federica Negro, and Giovanni Camussi

17. Extracellular Vesicle Loading via pH-Gradient Modification

            Stephanie M. Kronstadt, Steven M. Jay, and Anjana Jeyaram

Includes cutting-edge techniques

Provides step-by-step detail essential for reproducible results

Contains key implementation advice from the experts