Essentials of nucleic acid analysis

This book sets out to guide the analyst through the steps needed to obtain good quality results in DNA analysis. The underlying principles for achieving this goal were formulated by LCG as the six principles for ensuring valid analytical measurement, which are detailed in the introduction. The authors of each chapter are practitioners of the art of DNA analysis in areas where the quality of the result is critical. At a time were the use of DNA and nucleic acid analysis is growing exponentially, this title will provide an indispensable handbook and reference of the highest standard for those working in the fields of food analysis and forensic applications.

Valid Analytical Molecular Biology: The Challenge. Quality in the Analytical Molecular Biology Laboratory. An Introduction to Method Validation. DNA Extraction. DNA Quantification. PCR: Factors Affecting Reliability and Validity. Quantitative Real-time PCR Analysis. Multiplex PCR and Whole Genome Amplification. Procedures for Quality Control of RNA Samples for Use in Quantitative Reverse Transcription PCR. Microarrays.