Epithelial Cell Culture (2nd Ed., 2nd ed. 2024) Methods and Protocols Methods in Molecular Biology Series, Vol. 2749
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This second edition volume expands on the previous edition with in-depth discussions on the rapid advancements in epithelial cell biology, and the cutting-edge research and techniques used by researchers in the field. The chapters in this book cover topics such as detailed methodologies applicable to epithelial cells derived from primates, pigs, bovines, and laboratory animals; the manipulation and differentiation of epithelial cells; and epithelial cell models in the gastroenteric system in human medicine and nutrition. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and cutting-edge, Epithelial Cell Culture: Methods and Protocols, Second Edition is a valuable resource for researchers in the scientific community, educators, and students who are interested in unraveling the complexities of epithelial cell biology, cultivating curiosity, and inspiring the next generation of groundbreaking research.
Preface...
Table of Contents...
Contributing Authors...
1. Culture of Mouse Thymic Epithelial Cells in Serum-Free Medium
Yasuhiro Adachi
2. Cultured Pig Thyroid Follicular Cells: Electrical Evaluation of Epithelial Integrity
Peying Fong
3. Human Minor Salivary Glands: A Readily-Available Source of Salivary Stem/Progenitor Cells for Regenerative Applications
Caitlynn M. L. Barrows, Danielle Wu, Simon Young, and Mary C. Farach-Carson
4. Salivary Organotypic Tissue Culture: An Ex-Vivo 3D Model for Studying Radiation-Induced Injury of Human Salivary Glands
Akshaya Upadhyay, Migmar Tsamchoe, and Simon D. Tran
5. Differentiation of Pig Gastric Primary Cells into Mucus Producing Epithelial Cells
Macarena P. Quintana-Hayashi, Sinan Sharba, and Sara K. Lindén
6. Isolation, Culture, and Microscopic Imaging of Guinea Pig Primary Gastric Tissue Cells
Weronika Gonciarz and Magdalena Chmiela
7. Method for Two-Dimensional Epithelial Monolayer Formation Derived from Mouse Three-Dimensional Small Intestinal Organoids
Yuta Takase and Toshio Takahashi
8. Human Hepatic Spheroid Co-Culture Model for Assessment of Drug-Induced Liver Injury
Linhao Li and Hongbing Wang
9. In Vitro Porcine (Explant) Colon Culture
Matheus de Oliveira Costa and Michael K. Dame
10. Bioencapsulation of Oocytes and Granulosa Cells
Massimo Faustini, Stella Agradi, Daniele Vigo, Maria L. Torre, and Giulio Curone
11. Culturing and Differentiation of Patient-Derived Ectocervical Epithelial Stem Cells using Air-Liquid Interphase and Matrigel Scaffold
Rajendra Kumar Gurumurthy, Naveen Kumar, and Cindrilla Chumduri
12. Ovine Trophoblast Cells: Cell Isolation and Culturing from the Placenta at the Early Stage of Pregnancy
Paola Toschi, Irene Viola, Isabella Manenti, Silvia Miretti, Elisabetta Macchi, Eugenio Martignani, Paolo Accornero, and Mario Baratta
13. Amniotic Membrane and Amniotic Epithelial Cell Culture
Angelo Canciello, Adrian Cerveró-Varona, Maura Turriani, Valentina Russo, and Barbara Barboni
14. Evaluation of the Epithelial Barrier Integrity in Primary Cultures of Pig Mammary Epithelial Cells
Chiara Bernardini, Debora La Mantia, and Monica Forni
15. Bovine Skeletal Muscle Satellite Cells: Isolation, Growth, and Differentiation
Silvia Miretti, isabella Manenti, Paola Toschi, Elisabetta Macchi, Eugenio Martignani, Paolo Accornero, and Mario Baratta
16. Equine Induced Pluripotent Stem Cell Culture
Julia Falk and F. Xavier Donadeu
Subject Index List...
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Date de parution : 12-2023
Ouvrage de 186 p.
17.8x25.4 cm
