Epidermal Cells (3rd Ed., Softcover reprint of the original 3rd ed. 2014) Methods and Protocols Methods in Molecular Biology Series, Vol. 1195
Coordonnateur : Turksen Kursad
Reflecting over three decades of advances, Epidermal Cells: Methods and Protocols, Third Edition underscores these advances in our understanding of epidermal biology with updated and entirely new protocols that compliment and extend the earlier edition. The inclusion of protocols useful for both in vitro and in vivo studies reflects many useful developments in the field. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.
Dependable and easy to follow, Epidermal Cells: Methods and Protocols, Third Edition serves researchers working to accelerate the work in this vital field of study.
1. Differentiation of Human Induced Pluripotent Stem Cells into a Keratinocyte Lineage
Igor Kogut, Dennis R. Roop, and Ganna Bilousova
2. Differentiation of Epidermal Keratinocytes from Human Embryonic Stem Cells
Fahad K. Kidwai, Tong Cao, and Kai Lu
3. Protocol for Serial Cultivation of Epithelial Cells Without Enzymes or Chemical Compounds
Dongxia Ye and Antonio Peramo
4. Growth and Differentiation of HaCaT Keratinocytes
Van G. Wilson
5. Transgene Delivery to Cultured Keratinocytes Via Replication-Deficient Adenovirus Vectors
Vincent P. Ramirez and Brian J. Aneskievich
6. Analyzing the Global Chromatin Structure of Keratinocytes by MNase-Seq
Jason M. Rizzo and Satrajit Sinha
7. Analysis and Meta-Analysis of Transcriptional Profiling in Human Epidermis
Claudia Mimoso, Ding-Dar Lee, Jiri Zavadil, Marjana Tomic-Canic, and Miroslav Blumenberg
8. Compound Screening and Transcriptional Profiling in Human Primary Keratinocytes: A Brief Guideline
Raphaela Rid, Harald Hundsberger, and Kamil Önder
9. Preparation of Primary Cultures of Mouse Epidermal Keratinocytes and the Measurement of Phospholipase D Activity
Lakiea J. Bailey, Vivek Choudhary, Purnima Merai, and Wendy B. Bollag
10. Lipid Rafts and Detergent-Resistant Membranes in Epithelial Keratinocytes
Kathleen P. McGuinn and Mỹ G. Mahoney
11. MMP-2, 9 and TIMP-1, 2 Assays in Keratinocyte Cultures
Takashi Kobayashi
12. Reactive Oxygen Species (ROS) Protection Via Cysteine Oxidation in the Epidermal Cornified Cell Envelope
Wilbert P. Vermeij and Claude Backendorf
13. Modified Methods for Growing 3-D Skin Equivalents: An Update
Rebecca Lamb and Carrie A. Ambler
14. A Novel Three-Dimensional Cell Culture Method to Analyze Epidermal Cell Differentiation In Vitro
Yoji Okugawa and Yohei Hirai
15. Reconstruction of Normal and Pathological Human Epidermis on Polycarbonate Filter
Evelyne De Vuyst, Céline Charlier, Séverine Giltaire, Valérie De Glas, Catherine Lambert de Rouvroit, and Yves Poumay
16. Methods for the Preparation of an Autologous Serum-Free Cultured Epidermis and for Autografting Applications
John J. Wille, Jeremy J. Burdge, and Jong Y. Park
17. Human Keratinocyte Cultures in the Investigation of Early Steps of Human Papillomavirus Infection
Laura M. Griffin, Louis Cicchini, Tao Xu, and Dohun Pyeon
18. Preparation and Delivery of 4-Hydroxy-Tamoxifen for Clonal and Polyclonal Labeling of Cells of the Surface Ectoderm, Skin, and Hair Follicle
Christine Chevalier, Jean-François Nicolas, and Anne-Cécile Petit
19. Microdissection and Visualization of Individual Hair Follicles for Lineage Tracing Studies
Inês Sequeira, Emilie Legué, Suzanne Capgras, and Jean-François Nicolas
20. Isolation and Characterization of a Stem Cell Side-Population from Mouse Hair Follicles
Paula L. Miliani de Marval, Sun Hye Kim, and Marcelo L. Rodriguez Puebla
21. Multi-Scale Mathematical Modeling and Simulation of Cellular Dynamical Process
Shinji Nakaoka
Includes cutting-edge methods and protocols on epidermal cell research
Fully updated from the previous editions
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Date de parution : 08-2016
Ouvrage de 296 p.
17.8x25.4 cm
Disponible chez l'éditeur (délai d'approvisionnement : 15 jours).
Prix indicatif 116,04 €
Ajouter au panierDate de parution : 07-2014
Ouvrage de 296 p.
17.8x25.4 cm
Disponible chez l'éditeur (délai d'approvisionnement : 15 jours).
Prix indicatif 158,24 €
Ajouter au panier