Cell-Secreted Vesicles, 1st ed. 2023 Methods and Protocols Methods in Molecular Biology Series, Vol. 2668
Coordonnateur : Vainio Seppo
Part I: Purification and Analysis of Single or Multiple EVs
1. Plasmon-Enhanced Characterization of Single Extracellular Vesicles
Mi Ho Jeong, Taehwang Son, and Hyungsoon Im
2. Detection of Cell-Derived Exosomes via Surface-Enhanced Raman Scattering Using Aggregated Silver Nanoparticles
Yang Li, Yunpeng Wang, Jinwei Tian, and Jian-An Huang
3. In Vivo Analysis of Heterogeneous Extracellular Vesicles Using a Red-Shifted Bioluminescence Resonance Energy Transfer Reporter Protein
Gloria I. Perez, Michael H. Bachmann, and Masamitsu Kanada
4. Characterization of Extracellular Vesicles by Transmission Electron Microscopy and Immunolabelling Electron Microscopy
Maribel Lara Corona, Ilse Hurbain, Graça Raposo, and Guillaume Van Niel
5. Extracellular Vesicles Isolation by a Tangential-Flow-Filtration-Based Large-Scale Purification Method
Runjie Yuan, Yijun Zhou, Gabriel F. Arias, and Dirk P. Dittmer
6. Metabolomics Analysis of Urinary Extracellular Vesicles by Nuclear Magnetic Resonance and Liquid Chromatography-Mass Spectrometry
Marta Martin-Lorenzo, Dolores Molero, Gloria Alvarez-Llamas
7. Methodologies for Scalable Production of High Quality Purified Small Extracellular Vesicles from Conditioned Medium
Gabriella Andriolo, Elena Provasi, Andrea Brambilla, Stefano Panella, Sabrina Soncin, Viviana Lo Cicero, Marina Radrizzani, Lucia Turchetto, and Lucio Barile
8. Automated Online Isolation and Fractionation Method for Subpopulations of Extracellular Vesicles
Susanne K. Wiedmer, Evgen Multia, Thanaporn Liangsupree, and Marja-Liisa Riekkola
Part II: Targeting Cell Behavior Functions of EVs
9. A Novel Assay for Investigating the Role of Exosomes in Tumor Cell-Endothelial Cell Crosstalk
Yan Qiu, Wenli Jiang, and Ye Zeng
10. Nanoparticle (NP) Loading by Direct Incubation with Extracellular Vesicles-Secretor Cells: NP Encapsulation and Exosome Characterization
Ana Martín-Pardillos and Pilar Martín-Duque
11. Two Complementary Strategies to Quantitate Extracellular Vesicles Uptake Using Bioluminescence and Non-Lipidic Dyes
Víctor Toribio and María Yáñez-Mó
12. Integrin-Mediated Exosomal Homing to Organs
Eun Jeong Park and Motomu Shimaoka
13. Methods and Protocols for Using Extracellular Vesicles as Delivery Vehicles in Neuronal Research
Manjusha Vaidya, Nasif Sayeed, Caroline Hobson, Sandeep Sreerama, Jonhoi Smith, Riya Shah, and Kiminobu Sugaya
14. Quantitative Analysis of Extracellular Vesicle Release Using Artificial MicroRNAs
Albert Lu
Part III: Systems and Models to Study EVs
15. Purification of Bacterial-Enriched Extracellular Vesicle Samples from Feces by Density Gradient Ultracentrifugation
Nadiya Byts, Olha Makieieva, Artem Zhyvolozhnyi, Genevieve Bart, Johanna Korvala, Jenni Hekkala, Sonja Salmi, Anatoliy Samoylenko, and Justus Reunanen
16. Isolation and Characterization of Extracellular Vesicles from Lymphocytes
Lujain Al-Ghadir and Zhi Chen
17. Biogenesis of Mesoporous Silica Nanoparticles Enclosed in Extracellular Vesicles by Mouse Renal Adenocarcinoma Cells
Feby Wijaya Pratiwi, Keerthanaa Balasubramanian Shanthi, Olha Makieieva, Zih An Chen, Artem Zhyvolozhnyi, Ilkka Miinalainen, Genevieve Bart, Anatoliy Samoylenko, and Si-Han Wu
18. Magnetic Separation of Cell-Secreted Vesicles with Tailored Magnetic Particles and Downstream Applications
Mireia Bernuz, Arnau Pallarès-Rusiñol, Rosanna Rossi, Carolina Fernández-Senac, Mercè Martí, and María Isabel Pividori
19. Cilia-Derived Extracellular Vesicles in Caenorhabditis elegans: In Vivo Imaging and Quantification of Extracellular Vesicles Release and Capture
Adrià Razzauti, Teresa Lobo, and Patrick Laurent
20. Exosome-Based COVID-19 Vaccine
Jaeyoung Kim and Nikita Thapa
Includes cutting-edge techniques
Provides step-by-step detail essential for reproducible results
Contains key implementation advice from the experts
Date de parution : 05-2023
Ouvrage de 315 p.
17.8x25.4 cm
