Cell Migration in Three Dimensions, 1st ed. 2023 Methods in Molecular Biology Series, Vol. 2608

1. Cell Migration in Three Dimensions

            Coert Margadant

Part I: The Cell Biology of Cell Migration

2. Use of Ecto-Tagged Integrins to Monitor Integrin Exocytosis and Endocytosis

            Clotilde Huet-Calderwood, Felix Rivera-Molina, Derek Toomre, and David A. Calderwood

3. Probing the ER-Focal Adhesion Link during Cell Migration

            Noemi Antonella Guadagno and Cinzia Progida

4. Mapping the Localization of Proteins within Filopodia Using FiloMap

            Guillaume Jacquemet

5. Extended Methods for 2D Confinement

            Juan Manuel García-Arcos, Kevin Gateau, Larisa Venkova, and Matthieu Piel

6. Visualization of Exosome Release and Uptake during Cell Migration Using the Live Imaging Reporter pHluorin_M153R-CD63

            Bong Hwan Sung and Alissa M. Weaver

7. Approaches to Determine Nuclear Shape in Cells during Migration through Collagen Matrices

            Martin Svoren, Elena Camerini, Merijn van Erp, Feng Wei Yang, Gert-Jan Bakker, and Katarina Wolf

Part II: Developmental Model Systems to Assess Cell Migration during Morphogenesis

8. Dissecting Collective Cell Behavior in Migrating Testis Myotubes in Drosophila

            Maik C. Bischoff and Sven Bogdan

9. Whole Mount In Situ Hybridization for Detection of Migrating Zebrafish Endodermal Cells

            Antonius L. van Boxtel

10. Live Imaging Analysis of Epithelial Zippering during Mouse Neural Tube Closure

11. Time-Lapse Imaging and Morphometric Analysis of Tracheal Development in Drosophila

            Sofia J. Araújo and Marta Llimargas

12. A Guide Toward Multi-Scale and Quantitative Branching Analysis in the Mammary Gland

            Edouard Hannezo and Colinda L.G.J. Scheele

13. Analysis of Integrin-Dependent Melanoblast Migration during Development

            Amanda Haage and Guy Tanentzapf

Part III: Cell Migration in Cancers and the Tumor Microenvironment

14. Invadopodia Methods: Detection of Invadopodia Formation and Activity in Cancer Cells Using Reconstituted 2D and 3D Collagen-Based Matrices

            David Remy, Anne-Sophie Macé, Philippe Chavrier, and Pedro Monteiro

15. Analysis of Energy-Driven Leader-Follower Hierarchy during Collective Cancer Cell Invasion

            Jian Zhang and Cynthia A. Reinhart-King

16. Visualizing and Quantifying mRNA Localization at the Invasive Front of 3D Cancer Spheroids

            Konstadinos Moissoglu, Stephen J. Lockett, and Stavroula Mili

17. Multimodal Techniques to Study Tumor Growth, Basement Membrane Breaching, and Invasion in 3D Matrices

            Daan Smits and Antoine A. Khalil

18. Analysis of Collective Migration Patterns within Tumors

            Ralitza Staneva and Andrew G. Clark

19. An In Vivo Model to Study Cell Migration in XYZ-T Dimension Followed by Whole-Mount Re-Evaluation

            Ann L.B. Seynhaeve and Timo L.M. ten Hagen

Part IV: Blood Vessel Formation and Interactions

20. Angiogenesis Invasion Assay to Study Endothelial Cell Invasion and Sprouting Behavior

            Yuechao Dong, Florian Alonso, Tiya Jahjah, Isabelle Fremaux, and Elisabeth Génot

21. Live-Cell Labeling and Artificial Intelligence Approaches for High-Resolution XYZT Imaging of Cytoskeletal Dynamics during Collective Cell Migration

            Maxime Cammeraat, Marko Popovic, Wendy Stam, and Coert Margadant

22. Analysis of mRNA Subcellular Distribution in Collective Cell Migration

23. A Bio-Engineered Model for Studying Vascular-Pericyte Interactions of the Placenta

            Marta Cherubini and Kristina Haase

24. Analysis of Vascular Morphogenesis in Zebrafish

            Marvin Hubert and Wiebke Herzog

25. Analysis of Monocyte Recruitment during Inflammation by Intravital Imaging

            Jose M. Gonzalez-Granado, Alberto Del Monte, Laura Piqueras, Vicente Andres, and Cristina Rius

Includes cutting-edge techniques

Provides step-by-step detail essential for reproducible results

Contains key implementation advice from the experts