Recombinant Virus Vaccines, 1st ed. 2017 Methods and Protocols Methods in Molecular Biology Series, Vol. 1581

This volume provides readers with methods and protocols for understanding the development of recombinant viruses and their use as vaccines platforms. Recombinant Virus Vaccines: Methods and Protocols details the use of recombinant vaccines that are employed to either produce immunogens in vitro or elicit antibody production in vivo. The chapters in this book are divided into four parts: Part I explores double-stranded DNA viruses; Part II discusses negative sense single-stranded RNA viruses; Part III talks about positive sense single-stranded RNA viruses; and Part IV describes bacteriophages. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and cutting-edge, Recombinant Virus Vaccines:Methods and Protocols is a valuable resource for scientists and clinicians who are interested in learning about and adopting methods for use in basic and biomedical research directed toward generating and developing recombinant viral vaccines.

Development of Novel Vaccines Against Infectious Diseases Based on Chimpanzee Adenoviral Vector.- Development of Recombinant Canarypox Viruses Expressing Immunogens.- Fowl Adenovirus-Based Vaccine Platform.- Development of Recombinant HSV-Based Vaccine Vectors.- Generating Recombinant Pseudorabies Virus for Use as a Vaccine Platform.- Generation and Production of Modified Vaccinia Virus Ankara (MVA) as a Vaccine Vector.- Poxvirus Safety Analysis in the Pregnant Mouse Model, Vaccinia and Raccoonpox Viruses.- Development of Recombinant Arenavirus-Based Vaccines.- Development of Recombinant Measles Virus-Based Vaccines.- Recombinant Tri-Segmented Pichinde Virus as a Novel Live Viral Vaccine Platform.- Human Rhinovirus-A1 as an Expression Vector.- Generating Recombinant Vesicular Stomatitis Viruses for Use as Vaccine Platforms.- Alphavirus-Based Vaccines.- Display of HIV-1 Envelope Protein on Lambda Phage Scaffold as a VaccinePlatform.- Bacteriophage T4 as a Nanoparticle Platform to Display and Deliver Pathogen Antigens: Construction of an Effective Anthrax Vaccine.