New Technologies for Glutamate Interaction, 1st ed. 2024 Neurons and Glia Neuromethods Series, Vol. 2780

This volume explores the latest technologies used to study the co-existence of neuronal and neuron-glia synapses in the brain. The chapters in this volume are organized into three parts. Part One covers recent advancements in the technical approaches for studying glutamatergic signalling between neurons. Part Two highlights approaches to study the functional role of astrocytes at neuronal synapses. Part Three focuses on fast signalling at neuron-glia synapses, and highlights relevant methods for investigating these unusual synapses, including slice electrophysiology and in vivo gene delivery techniques. In the Neuromethods series style, all chapters contain key advices from experts in the field that are valuable for performing successful experiments on the topic in your laboratory.

Cutting-edge and thorough, New Technologies for Glutamate Interactions: Neurons and Glia is a valuable resource for both neuronal and glial physiologists who are interested in learning about and developing new methods to further understand neuronal and neuron-glia synaptic transmission.

Series Preface…
Preface…
Table of Contents…
Contributing Authors…

Part I New Approaches to Study Glutamatergic Synapses between Neurons

1.      Visualization of Glutamatergic Neurotransmission in Diverse Model Organisms with Genetically Encoded Indicators

2.      Surface Glutamate Receptors Nanoscale Organization with Super-Resolution Microscopy (dSTORM)
Joana Ferreira and Laurent Groc

3.      Ligand-Directed Chemical Labeling for Visualizing and Analyzing AMPA Receptors in Neurons

Shigeki Kiyonaka, Kyohei Soga, Kento Ojima, Hiroshi Nonaka, and Itaru Hamachi

4.      Live FRET-FLIM Imaging to Study Metabotropic Signaling via the NMDA Receptor

Mehreen Manikkoth and Kim Dore

5.      Quantitative Analysis of Single Glutamatergic Vesicles in the Brain

Yuanmo Wang, Ajay Pradhan, Pankaj Gupta, Hanna Karlsson-Fernberg, and Ann-Sofie Cans

6.      Imaging Microtubule Network in Rodent Giant Glutamatergic Presynaptic Terminals

Laurent Guillaud

7.      Photooxidation of Genetically Encoded MiniSOG-Fused VGLUT2 for Identification of Glutamatergic Synapses by Transmission and 3D Electron Microscopy

Andrew J. Flores, Anna Nilsson, Junru Hu, Mason R. Mackey, Mark H. Ellisman, Thomas S. Hnasko, and Daniela Boassa

Part II New Approaches to Study Role of Astrocytes at Neuronal Synapses

8.      Chemogenetic Approaches to Study Astrocytes at Glutamatergic Synapses

9.      Studying the Role of Astrocytes at Synapses using Single-Cell Transcriptomics

Francisco Pestana, T. Grant Belgard, Thierry Voet, and Matthew G. Holt

10.  Analysis of Synaptic Glutamate Clearance as a Possible Indicator of Synaptic Health in the Degenerating Rodent Brain

Anton Dvorzhak and Rosemarie Grantyn

11.  Computational Models of Astrocyte Function at Glutamatergic Synapses

Kerstin Lenk, Audrey Denizot, Barbara Genocchi, Ippa Seppälä, Marsa Taheri, and Suhita Nadkarni

Part III Technical Approaches to Study Fast Signaling at Neuron-Glia Synapses

12.  Electrophysiological Recordings of Oligodendroglia in Adult Mouse Brain Slices

13.  Synaptic Integration at Neuron-OPC Synapses

Wenjing Sun

14.  Mapping Synaptic Inputs to Oligodendroglial Cells using In Vivo Monosynaptic Viral Tracing

Belgin Yalçın and Michelle Monje

15.  In Vivo Viral Gene Delivery to Manipulate Functional Properties of AMPA Receptors in Oligodendrocyte Lineage Cells

Ting-Jiun Chen, Bartosz Kula, and Maria Kukley

16.  Studying Synaptic Integration of Glioma Cells into Neural Circuits

Kiarash Shamardani, Kathryn R. Taylor, Tara Barron, and Michelle Monje

Subject Index List… 

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts