Macrophage Migration Inhibitory Factor, 1st ed. 2020 Methods and Protocols Methods in Molecular Biology Series, Vol. 2080
Coordonnateurs : Harris James, Morand Eric F.
This volume looks at--and discusses the techniques of--a range of areas in biology in which macrophage migration inhibitory factor (MIF) is studied. Topics covered in this book include methods to quantify MIF in cell supernatants and human samples, using ELISA and western blot; detection and analysis of intracellular MIF using flow cytometry; genotyping of human MIF polymorphisms, detection of MIF expression in a marine invertebrate using rapid amplification of cDNA RACE; studying the MIF receptor CD74 in tissues using immunohistochemistry; and studying MIF in models of disease such as asthma, Plasmodium infection, and gout. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and practical, Macrophage Migration Inhibitory Factor: Methods and Protocols is a valuable resource that will help researchers gain a new understanding of MIF biology in health and disease.
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Date de parution : 11-2020
Ouvrage de 265 p.
17.8x25.4 cm
Disponible chez l'éditeur (délai d'approvisionnement : 15 jours).
Prix indicatif 116,04 €
Ajouter au panierDate de parution : 11-2019
Ouvrage de 265 p.
17.8x25.4 cm
Disponible chez l'éditeur (délai d'approvisionnement : 15 jours).
Prix indicatif 105,49 €
Ajouter au panierThèmes de Macrophage Migration Inhibitory Factor :
Mots-clés :
Cell death; CRISPR; siRNA; autophagy; Mass spectrometry; genotyping