Immunohistochemistry and Immunocytochemistry, 1st ed. 2022 Methods and Protocols Methods in Molecular Biology Series, Vol. 2422
This volume provides a comprehensive reference guide for researchers to study the applications of labeled antibodies. Chapters guide reader through the theory and practice of immunohistochemistry, immunocytochemistry and immunofluorescence techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and useful tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Immunohistochemistry and Immunofluorescence: Methods and Protocols aims to be a useful practical guide to scientists to help further their study in this field
1. Introduction to Immunohistochemistry. From Evolving Science to Timeless Art
Luis Del Valle
2. Immunohistochemistry in Historical Perspective. Knowing the Past to Understand the Present
Carlos Ortiz-Hidalgo
3. Sample Preparation & Fixation for Histology and Pathology
Enrique Agustín Sampedro Carrillo
4. Tissue Processing
Sarah J. Aziz, and Cady E. Zeman-Pocrnich
5. Antigen Retrieval and Signal Amplification
Logan Dunkenberger, and Luis Del Valle
6. Primary Antibody Selection and Blocking Techniques for Immunohistochemistry
Lori A. Peton, and Miguel Reyes-Múgica
7. Probes for Fluorescent Visualization of Specific Cellular Organelles
Timothy Paul Foster
8. Enzymatic Immunohistochemistry
Rodolfo Henrich Lobo
9. Fluorescent Immunohistochemistry
Vanessa Moreno, Elizabeth A. Smith, and Sergio Piña-Oviedo
10. Double Labeling Fluorescent Immunocytochemistry
Monika Rak, and Krzysztof Reiss
11. Multiplexing and Spectral Microscopy
Logan Dunkenberger, Adriana Zapata, and Luis Del Valle
12. Fluorescent In situ Hybridization (FISH)
Jeanette M. Ramos
13. Proximity Ligation Assay (PLA)
Muhammad S. Alam
14. Immunohistochemistry, Quality control and Principles of Validation in the Central Nervous System
Verónica Velasco-Vales, Danny Soria-Céspedes, Teresa Cristina Cuesta-Mejías, and Norma Cecilia Padrón-Pérez
15. Culture and Phenotyping of Glial Cell Cultures, Gliospheres and Neurospheres.
Amanda Parker-Struckhoff, Krzysztof Reiss, and Luis Del Valle
16. ER+ Breast Cancer Mammosphere Formation and Analysis
Ciera S. Singleton, Leo Li-Ying Chan, Kelsey J. McCulley, Sarah L. Kessel, Luis Del Valle, and Judy S. Crabtree
17. Detection of Circulating and Tissue Myeloid-Derived Suppressor Cells (MDSCs) by Flow Cytometry
Maria Dulfary Sanchez-Pino
18. Comet Assay for the Detection of Single and Double Strand DNA Breaks
Logan Dunkenberger, Krzysztof Reiss, and Luis Del Valle
19. Fluorescent-Activated Cell Sorting (Flow Cytometry)
Luis F. Carrillo
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Date de parution : 12-2022
Ouvrage de 286 p.
17.8x25.4 cm
Date de parution : 12-2021
Ouvrage de 286 p.
17.8x25.4 cm
