Cancer Stem Cells, 1st ed. 2018 Methods and Protocols Methods in Molecular Biology Series, Vol. 1692

1. Introduction to Cancer Stem Cells: Past, Present, and Future

            David Bakhshinyan, Ashley A. Adile, Maleeha A. Qazi, Mohini Singh, Michelle M. Kameda-Smith, Nick Yelle, Chirayu Chokshi, Chitra Venugopal, and Shiela K. Singh

2. Surface Markers for the Identification of Cancer Stem Cells

            Vinod Gopalan, Farhadul Islam, and Alfred King-yin Lam

3. The Role of CD44 and Cancer Stem Cells

            Liang Wang, Xiangsheng Zuo, Keping Xie, and Daoyan Wei

4. Evaluation and Isolation of Cancer Stem Cells Using ALDH Activity Assay

            Luigi Mele, Davide Liccardo, and Virginia Tirino

5. Isolation of Cancer Stem Cells by Side Population Method

            Masayuki Shimoda, Masahide Ota, and Yasunori Okada

6. Self-Renewal and CSCs In VitroEnrichment: Growth as Floating Spheres

            Pooja Mehta, Caymen Novak, Shreya Raghavan, Maria Ward, and Geeta Mehta

7. In Vitro Tumorigenic Assay: The Tumor Spheres Assay

            Hui Wang, Anna Paczulla, Martina Konantz, and Claudia Lengerke

8. In Vitro Tumorigenic Assay: Colony Forming Assay for Cancer Stem Cells

9. Xenograft as In Vivo Experimental Model

            Manuela Porru, Luca Pompili, Carla Caruso, and Carlo Leonetti

10. How to Assess Drug Resistance in Cancer Stem Cells

            Maria Laura De Angelis, Ruggero De Maria, and Marta Baiocchi

11. Tumor Tissue Analogs for Assessment of Radioresistance in Cancer Stem Cells

            Meenakshi Upreti

12. Generation of In Vitro Model of Epithelial Mesenchymal Transition (EMT) Via the Expression of a Cytoplasmic Mutant Form of Promylocytic Leukemia Protein (PML)

            Anna Di Biase, Amanda K. Miles, and Tarik Regad

13. Identification and Isolation of Cancer Stem Cells Using NANOG-EGFP Reporter System

            Magdalena E. Buczek, Stephen P. Reeder, and Tarik Regad

14. Determination of miRNAs from Cancer Stem Cells Using a Low Density Array Platform

            Hiromichi Kawasaki, Angela Lombardi, and Michele Caraglia

15.Assessing DNA Methylation in Cancer Stem Cells

16. Histones Acetylation and Cancer Stem Cells (CSCs)

            Vivian Petersen Wagner, Manoela Domingues Martins, and Rogerio Moraes Castilho

17. Immunohistochemistry for Cancer Stem Cells Detection: Principles and Methods

            Martina Intartaglia, Rosalaura Sabetta, Monica Gargiulo, Giovanna Roncador, Federica Zito Marino, and Renato Franco

18. Circulating Tumor Cells

            Sebastián A. García, Jürgen Weitz, and Sebastian Schölch

Includes cutting-edge techniques for the study of cancer stem cells

Provides step-by-step detail essential for reproducible results

Contains key implementation advice from the experts