Biomedical Engineering Technologies, 1st ed. 2022 Volume 1 Methods in Molecular Biology Series, Vol. 2393

This volume provides detailed technical protocols on current biosensors and imaging technologies and Chapters focus on optical, electrochemical, Quartz crystal microbalance (QCM) biosensors and on medical imaging technologies such as tomography, MRI, and NMR. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

Authoritative and practical, Biomedical Engineering Technologies, Volume 1 provides technical details in descriptions of major technologies by experts in the field. 

Part I: Optical

1. A Compact Surface Plasmon Resonance Biosensor for Sensitive Detection of Exosomal Proteins for Cancer’

Diagn Yun Wu, Xie Zeng, and Qiaoqiang Gan

2. Electrochemistry Coupling Localized Surface Plasmon Resonance for Biochemical Detection

Zetao Chen, Yanli Lu, Qingqing Zhang, Diming Zhang, Shuang Li, and Qingjun Liu

3. Preparation of surface plasmon resonance aptasensor for human activated protein C sensing

Semra Akgönüllü, Seda Koyun, Handan Yavuz, Arzum Erdem, and Adil Denizli

4. Large-scale nanogrooved photonic crystals for label-free biosensing by guided-mode resonance

Miquel Avella-Oliver, Gabriel Sancho-Fornes, Rosa Puchades, and Ángel Maquieira

Amogha Tadimety, Yichen Zhang, John H. Molinski, Timothy J. Palinski, Gregory J. Tsongalis, and John X.J. Zhang

6. Ultrasensitive Label-Free Nucleic-Acid Biosensors Based on Bimodal Waveguide Interferometers

Cesar S. Huertas, and Laura M. Lechuga

7. Azimuthal Beam Scanning Microscope Design and Implementation for Axial Localization with Scanning Angle Interference Microscopy

Marshall Colville, Sangwoo Park, Avtar Singh, Matthew Paszek, and Warren Zipfel

8. Computational Optics for Point-of-Care Breast Cancer ProfilingJouha

Min, Matthew Allen, Cesar M. Castro, Hakho Lee, Ralph Weissleder, and Hyungsoon Im

9. DNA genotyping based on isothermal amplification and colorimetric detection by consumer electronics devices, Luis Antonio Tortajada-Genaro

 Miguel R. Ossandon, Brian S. Sorg, Dhananjay S. Phatak, and Konstantinos Kalpakis

11. Automated multidimensional nanoscale chromatography for ultra-sensitive targeted mass spectrometry

Paolo Cifani, and Alex Kentsis

12. Clinical Translation of Stimulated Raman Histology

Cordelia Orillac, Todd Hollon, and Daniel A. Orringer

13. Ex vivo and in vitro methods for detection of bioactive staphylococcal enterotoxins

Reuven Rasooly, Paula Do, and Bradley Hernlem

14. An Ultracompact Real-time Fluorescence Loop-mediated Isothermal Amplification (LAMP) Analyzer, Gihoon Choi, and Weihua Guan

Jiasi Wang, Jason E. Kreutz, and Daniel T. Chiu

16. Sample-to-Answer Microfluidic Nucleic Acid Testing (NAT) (file #on Lab-on-a-Disc for Malaria Detection at Point-of-Need

 Gihoon Choi, and Weihua Guan

17. Charge sensitive optical detection for measurement of small molecule binding kinetics

Shaopeng Wang, Guangzhong Ma, Runli Liang, and Nongjian Tao

18. Mobile Elisa With A Transparent Incubator

Arsenii Zhdanov, Cassidy Chen, Crystal Schmitt, Tyler Tripp, Chase Miller, and Anna Pyayt

19. Smartphone Enabled Point-of-Care Detection of Serum Biomarkers

 Jacob T. Heggestad, David S. Kinnamon, Jason Liu, Daniel Y. Joh, Cassio M. Fontes, Qingshan Wei, Aydogan Ozcan, Angus M. Hucknall, and Ashutosh Chilkoti

20. Micromachined Optical Fiber Sensors for Biomedical Applications

Chen Zhu, Rex E. Gerald II, and Jie Huang

PART II: Electrochemical and Piezoelectric

21. Optical and electrochemical aptasensors for sensitive detection of aflatoxin B1 and aflatoxin M1 in blood serum, grape juice and milk samples

Mohammad Ramezani, Seyed Hamid Jalaliana, Seyed Mohammad Taghdisi, Khalil Abnous, and Mona Alibolandi

22. DNA Generated Electric Current Biosensor for Epidermal Growth Factor Receptor 2 (HER2) Analysis,  Xiaoqing Li, Congcong Shen, Minghui Yang, and Avraham Rasooly

23. Agar-Integrated Three-Dimensional Microelectrodes for On-Chip Impedimetric Monitoring of Bacterial Viability

Derrick Butler, and Aida Ebrahimi

Fei Liu, Bin Wang, Yanke Shan, Wenjie Kang, Jiahao Li, Xin Wang, and Jing Liu

25. Electrochemical aptamer-based sensors: a platform approach to high frequency molecular monitoring in situ in the living body

Philippe Dauphin-Ducharme

Kyle L. Ploense, Netzahualcóyotl Arroyo-Currás, Tod E. Kippin, and Kevin W. Plaxco

26. Smartphone-based Electrochemical System for Biosensors and Biodetection

Daizong Ji, Sze Shin Low, Diming Zhang, Lei Liu, Yanli Lu, and Qingjun Liu

27. Preparation of notch-4 receptor containing quartz crystal microbalance biosensor for MDA MB 231 cancer cell detection

Monireh Bakhshpour, Ayse Kevser Piskin, Handan Yavuz, and Adil Denizli

28. The study of the skin cells viscoelastic changes using QCM-D measurements

Anna Sobiepanek, and Tomasz Kobiela

Part III: Monitoring and Imaging

29. Hyperpolarized micro-NMR platform for sensitive analysis of in vitro metabolic flux in living cells

Sangmoo Jeong, and Kayvan R. Keshari

30. Mixed Modeling Frameworks for Analyzing Whole-Brain Network Data

Sean L. Simpson

31. Rapid In Vivo Quantification of Creatine Kinase Activity by Phosphorous-31 Magnetic Resonance Spectroscopic Fingerprinting (31P-MRSF)

Charlie Wang, Kihwan Kim, and Xin Yu

32. Nanoparticle assisted nuclear relaxation-based detection of oligonucleotides

Rebecca N. Silva, and Peng Zhang

33. Imaging Biomarker Development for Lower Back Pain using Machine Learning

Bilwaj Gaonkar, Kirstin Cook, Bryan Yoo, Banafsheh Salehi, and Luke Macyszyn

34. Acoustic Angiography: Superharmonic Contrast-Enhanced Ultrasound Imaging for Non-Invasive Visualization of Microvasculature

Isabel G. Newsome, and Paul A. Dayton

35. Patch Clamp Technology for Focused Ultrasonic (FUS) (file #Neuromodulation

Eun Sok Kim

36. Wireless Wearable Ultrasound Sensor to Characterize Respiratory Behavior

Ang Chen, Rachel Diane Rhoades, Andrew Joshua Halton, Jayden Charles Booth, Xinhao Shi, Xiangli Bu, Ning Wu, and Junseok Chae

37. Forming large effective ultrasound arrays using the swept synthetic aperture technique

Nick Bottenus

38. Quantitative bioluminescence tomography for in vivo volumetric-guided radiotherapy

Zijian Deng, Xiangkun Xu, Hamid Dehghani, Daniel M). Sforza, Iulian Iordachita, Michael Lim, John W. Wong, and Ken Kang-Hsin Wang

39. Evaluation of pulmonary structure and function in patients with cystic fibrosis from electrical impedance tomography data

Jennifer L. Mueller

40. In-Vivo Quantitative Whole-Body Perfusion Imaging Using Radiolabeled Copper(II) (file #bis(thiosemicarbazone) (file #Complexes and Positron Emission Tomography (PET)

Mark A. Green, Carla J. Mathias, Nathaniel J. Smith, Monica Cheng, and Gary D. Hutchins

41. Biodegradable AuNP-based plasmonic nanogels as contrast agents for computed tomography and photoacoustics

Mathilde Bouché, and David P. Cormode

42. Cationic Contrast Agents for Computed Tomography of Cartilage for Early Diagnosis of Osteoarthritis

Chenzhen Zhang, Ambika G. Bajpayee, and Tengfei He

Jia Wang, and R. Michael van Dam

44. Preparation of radiolabeled antibodies for nuclear medicine applications in immuno-oncology

Junnian Wei, David Y. Oh, and Michael J. Evans

45. A Wireless Fully-Passive Acquisition of Biopotentials

Shiyi Liu, Xueling Meng, Jianwei Zhang, and Junseok Chae

46 . Fabrication of Skin-Mountable Flexible Sensor Patch for Monitoring of Swallowing Function Heun Park, Min Ku Kim, Georgia A). Malandraki, and Chi Hwan Lee

47. The Intelligent Phenotypic Plasticity Platform (IP3) (file #for Precision Medicine-based Injury Prevention in Sport

Adam W. Kiefer, Cortney N. Armitano-Lago, Anoop Sathyan, Ryan MacPherson, Kelly Cohen, and Paula L. Silva

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible result

Contains key notes and implementation advice from the experts