Microfluidics Diagnostics, 1st ed. 2024 Methods and Protocols Methods in Molecular Biology Series, Vol. 2804

Part I: Overview

1. Self-Powered Microfluidics for Point-of-Care Solutions: From Sampling to Detection of Proteins and Nucleic Acids

            Dries Vloemans, Lorenz Van Hileghem, Henry Ordutowski, Francesco Dal Dosso, Dragana Spasic, and Jeroen Lammertyn

Part II: Microchips for Sample Preparation Including Cell-Free DNA, Exosomes, and Cells

2. DNA Sizing with pg/mL Sensitivity for Cell-Free DNA Analysis with the mLAS Technology

            Audrey Boutonnet, Inga Tijunelyte, Aurélien Bancaud, and Frédéric Ginot

3. On-Chip Magnetic Extraction of Circulating Cell-Free DNA from Biological Samples

            Karla Perez-Toralla, Iago Pereiro, Sonia Garrigou, Fahima Di Faderico, Monica Araya-Farias, Charlotte Proudhon, François-Clémént Bidard, Jean-Louis Viovy, Valérie Talya, and Stéphanie Descroix

4. Isolation of Extracellular Vesicles from Cell Culture and Blood through Nano-Targeted DLD Microfluidic Device

            Marie Gaillard, Nicolas Sarrut-Rio, Catherine Pudda, François Boizot, Yoann Roupioz, and Aurélie Thuaire

5. Microchip for immunomagnetic sorting of Circulating Tumor Cells (CTCs)

Part III: Microchips for Protein Markers Analysis/Detection

6. Enhancing the Efficiency of Conventional Surface Immunoassays within Standard Labware Using Microscale Flows

            Iago Pereiro, Anna Fomitcheva-Khartchenko, and Govind V. Kaigala

7. Droplet Microfluidics with Capillary Electrophoresis for Glycan Biomarker Analysis

8. Microfluidic Cartridge for Bead-Based Affinity Assays

            Ines F. Pinto, Veronique Chotteau, and Aman Russom

Part IV: Microchips for Single Cell Analysis

9. A Guide to the Quantitation of Protein Secretion Dynamics at the Single-Cell Level

            Nathan Aymerich, Olivia T.M. Bucheli, Kevin Portmann, Klaus Eyer, and Jean Baudry

10. Magnetic Microtweezers for High-Throughput Bioseparation in Sub-Nanoliter Droplets

            Simon Dumas, Lucile Alexandre, Mathilde Richerd, Marco Serra, and Stéphanie Descroix

Part V: Microfluidic Chips for Analysis of Bacteria and Virus

11. A Thermoplastic Microsystem to Perform Antibiotic Susceptibility Testing by Monitoring Oxygen Consumption

            Petra Jusková, André Kling, Steven Schmitt, and Petra S. Dittrich

12. An Immiscible-Phase Filtration Device for Isolation, Amplification, and Detection of Nucleic Acids for Clinical Diagnostics

Part VI: Microfluidic Chips for Human Cell-Based Culture and Analysis

13. Microfluidic 3D Cytotoxic Assay

            Hyeri Choi, Sunghun Cheong, Ailian Jin, Dohyun Park, and Noo Li Jeon

14. Evaluating Nanoparticles Penetration by a New Microfluidic Hydrogel-Based Approach

            Saba Goodarzi, François Lux, and Charlotte Rivière

15. Selective Targeting of Tumor Cells in a Microfluidic Tumor Model with Multiple Cell Types

            Bas van de Crommert, Valentina Palacio-Castañeda, and Wouter P.R. Verdurmen

Includes cutting-edge techniques

Provides step-by-step detail essential for reproducible results

Contains key implementation advice from the experts