Planar Gel Electrophoresis For Protein Separation Handbooks in Separation Science Series

Planar Gel Electrophoresis: For Protein Separation presents the state-of-the-art planar gel electrophoretic techniques and how they can be implemented in laboratory practice for protein separation. It covers the whole of the subject, including the historical background, concepts, applications and new developments in planar gel electrophoresis in protein separation. The chapters are written by experts with deep experience in their field, and extensive selected referencing facilitates identification of key concepts out of the general background of information?contained in the current literature. This volume serves as a practical guide for researchers to choose and utilize suitable sample preparation procedures and describes notable contemporary uses in planar gel electrophoresis in industrial and academic project environments.

Section 1: History, Theory and Milestones  1. Historical backgrounds and milestones  2. Theory and core concepts  3. Different gel chemistry and buffer systems

Section 2: Sample preparation  4. Preparation of protein samples

Section 3: Different electrophoretic techniques  5. SDS-PAGE (Denaturing gels)  6. Non-denaturing gels  7. Non-reducing gels  8. Gradient gels to cover high MW and low MW proteins  9. Separation of low MW peptides with Tris-Tricine gels  10. Agarose Gels for Separating High MW Proteins  11. Preparative gels  12. Acid- urea – triton gel for separating baisc proteins and histones  13. Blue native gels for separating protein complex  14. Pore gradient gel electrophoresis (PGGE)  15. Cationic Electrophoresis  16. Isoelectric focusing (IEF)  17. Immobilized pH gradients (IPG)  18. Two-dimenstional gel electrophoresis (2-DE)  19. Difference gel electrophoresis (DIGE)  20. Diagonal Electrophoresis for disulfide bond detection  21. Affinity gel electrophoresis (AEP)  22. Electrophoretic mobility shift assays (EMSA)  23. Immobilized metal affinity electrophoresis (IMAEP)  24. 1D and 2D zymography (1DZ and 2DZ)  25. SARCOSYL-PAGE: separation of PEGylated proteins.  26. Phenylboronate acrylamide gel electrophoresis for glycated proteins  27. Field Inversion gel electrophoresis (FIGE)  28. Microfluidic gel electrophoresis

Section 4: Post-electrophoresis analysis  29. Visualization of gel separated proteins with protein stains  30. Densitometry analysis of protein bands or spots  31. Data analysis software for extracting information from gel separations  32. Recovery of proteins from gel with electroelution  33. In-gel proteolytic digestion of proteins and mass spectrometry

Section 5: Coulping electrophoresis with antibody detection  34. Western Blotting  35. Serological Proteome Analysis (SERPA)  36. Immunofixation Electrophoresis (IFE)  37. Immunoelectrophoresis (IEP)  

• Contains the latest developments and applications of planar gel electrophoresis for protein separation
• Guides scientists in choosing the appropriate techniques to use to solve their problems, with an emphasis on the biological functions of the studies
• Each chapter provides an overview of a single topic with a focus on the big picture as well as the most relevant information for project planning