Modern biophysical chemistry: detection and analysis of biomolecules

The only introductory text to address contemporary methods, the really interesting stuff to spice up a standard biophysics course is to be found here. Accessible and didactically written, it is based on a graduate course taught by the author for several years. By presenting a mix of basic theory and real life application examples, he successfully bridges the gap between theory and experiment. Divided into two major parts, this advanced textbook explains all relevant methods used in current industrial research. The first part, basic biophysical chemistry, surveys basic spectroscopic techniques and properties that are the prerequisite for the more sophisticated technologies discussed in the remainder of the book. The second part covers modern, cutting-edge bioanalytical techniques based on physical methods, such as confocal fluorescence, ultrafast spectroscopy, optical tweezers, single molecule electrical measurements, quantum dots, and single molecule force microscopy. Equally, any student contemplating a career in the chemical, pharmaceutical or bio-industry will greatly benefit from the technological knowledge presented.

Introduction: What is Biophysical Chemistry? PART 1: BASIC METHODS IN BIOPHYSICAL CHEMISTRY. Basic Optical Principles. Optical Properties of Biomolecules. Basic Fluorescence Techniques. Chiroptical and Scattering Methods. Magnetic resonance techniques. Mass spectrometry. PART 2: ADVANCED METHODS IN BIOPHYSICAL CHEMISTRY. Fluorescene Microscopy. Single-Biomolecule Techniques. Ultrafast and Nonlinear Spectroscopy. Special Techniques. Assay Development, Readers and High-Throughput Screening.

Master's Students in Biochemistry, Ph.D. Students in Biochemistry, Ph.D. Students in Chemistry, Ph.D. Students in Physics, Biotechnologists, Biophysicists