Lysosomes and Lysosomal Diseases Methods in Cell Biology Series
Auteur : PLATT Frances
Bethan S. Kilpatrick, Emily R. Eden, Leanne N. Hockey, Clare E. Futter and Sandip Patel
2. A Rapid Method for the Preparation of Ultra-pure, Functional Lysosomes Using Funcionalised Superparamagnetic Iron Oxide Nanoparticles
Mathew William Walker and Emyr Lloyd-Evans
3. TFEB and the CLEAR Network
Carmine Settembre and Diego L. Medina
4. Biosynthesis, Targeting and Processing of Lysosomal Proteins: Pulse-Chase Labeling and Immune Precipitation
Sandra Pohl and Andrej Hasilik
5. Measuring Lysosomal pH by Fluorescence Microscopy
Johnathan Canton and Sergio Grinstein
6. Lysosome Fusion in Cultured Mammalian Cells
Nicholas A. Bright, Lena Wartosch and J. Paul Luzio
7. RNAi Screens of Lysosomal Trafficking
Salil Garg, Michael B. Brenner
8. Approaches for Plasma Membrane Wounding and Assessment of Lysosome-mediated Repair Responses
Corrotte, M, Castro-Gomes, T, Koushik, A.B. and Andrews, N.W
9. Imaging Approaches to Measuring Lysosomal Calcium
Anthony J. Morgan, Lianne C. Davis, Antony Galione
10. Lysosome Electrophysiology
Xi Zoë Zhong and Xian-Ping Dong
11. Reconstitution of Lysosomal Ion Channels into Artificial Membranes
Elisa Venturi and Rebecca Sitsapesan
12. Fluorescence Methods for Analysis of Interactions Between Ca2+ Signalling, Lysosomes and Endoplasmic Reticulum
David L. Prole, Cristina I. López-Sanjurjo, Stephen C. Tovey and Colin W. Taylor
13. Methods for the Quantification of Lysosomal Membrane Permeabilization: a Hallmark of Lysosomal Cell Death
Sonja Aits, Marja Jäättelä and Jesper Nylandsted
14. Measuring the Phagocytic Activity of Cells
Nick Platt and Paul Fineran
15. Detection and Quantification of Microbial Manipulation of Phagosomal Function
Maria Podinovskaia, David G. Russell
16. Measuring Relative Lysosomal Volume for Monitoring Lysosomal Storage Diseases
Danielle te Vruchte, Kerri L. Wallom and Frances M. Platt
17. Quantifying Storage Material Accumulation in Tissue Sections
Jonathan D Cooper, Helen R Brooks, Hemanth R Nelvagal
18. Laboratory Diagnosis of Niemann-Pick disease Type C: The Filipin Staining Test
Marie T. Vanier and Philippe Latour
- Covers sections on model systems and functional studies, imaging-based approaches and emerging studies
- Chapters are written by experts in the field
- Cutting-edge material
Date de parution : 02-2015
Ouvrage de 450 p.
19x23.4 cm
Thème de Lysosomes and Lysosomal Diseases :
Mots-clés :
Acid sphingomyelinase; Antigen presentation; Assessing therapeutic efficacy; Autofluorescence; Autophagy; Bafilomycin A1; Biomarker; Biosynthesis; Ca2+Endoplasmic reticulum; Ca2+GPN; Ca2+-release; Calcium; Cathepsins; CD1; Cell injury; Cell wounding; Cellular assay; Cholesterol homeostasis; CLEAR network; Confocal microscopy; Content mixing; Death pathway; Dendritic cell; Disease progression; Dual-wavelength ratio imaging; Electron microscopy; Electrophysiology; Endocytosis; Endolysosome; Endosome; Exocytosis of lysosomes; Filipin; Flow cytometry; fluorescein isothiocyanate; Fluorescence microscopy; fluorescence microscopy; Fluorescence; Fluorescent proteins; Fluorogenic sensors; Galectins; High content screening; Immune precipitation; Immunofluorescence; Immunostaining; Inositol trisphosphate receptor; Intracellular cholesterol; Intracellular microbes; Intracellular trafficking; Ion channel reconstitution; Ion channel; Laboratory diagnosis; LAMP1; Late endosome; LMP; Lysosomal biogenesis; Lysosomal cell death; Lysosomal clearance; Lysosomal dysfunction; Lysosomal enzyme; Lysosomal exocytosis; Lysosomal membrane permeabilization; Lysosomal purification; Lysosomal storage disease; Lysosome function; Lysosome patch clamping; Lysosome trafficking; Lysosome; Lysosomes; LysoTracker; Macrophage; Membrane fusion; Membrane traffic; Metabolic labeling; Method; MHC II; Microscopy; NAADP; Niemann-Pick C disease; Nigericin; NPC1; NPC2; Optical microscopy; Particle; pH; Phagocyte; Phagocytosis; Phagosome maturation; Planar phospholipid bilayer; Plasma membrane repair; Plasma membrane resealing; Processing; Quantitative analysis; Relative lysosomal volume; RNAi screen; RNAi; Secretion of lysosomal enzymes; shRNA; Single channel; Storage material accumulation; Subcellular fractionation; Superparamagnetic nanoparticle; Targeting; TFEB methods; TFEB; Thresholding image analysis; TPC1; TPC2; Transcriptional regulation; Two-pore channels; Vesicle trafficking; Voltage-clamp
