Wheat Biotechnology, Softcover reprint of the original 1st ed. 2017 Methods and Protocols Methods in Molecular Biology Series, Vol. 1679

This detailed volume provides background on recent new technology developments highlighting the potential of the genomic era in wheat breeding with invaluable instruction on the methodology, which is complemented by overview chapters on the status of new technology application in major wheat production countries. The topics, addressed by internationally renowned scientists active in the field, cover methods underpinning the latest developments in the field of wheat biotechnology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Wheat Biotechnology: Methods and Protocols serves as a vital resource for scientists working to breed future high-yielding wheat varieties to sustain a growing population in an increasingly unpredictable world.

1. Enabling Molecular Technologies for Trait Improvement in Wheat

            Prem L. Bhalla, Akanksha Sharma, and Mohan B. Singh

2. What Will Be the Benefits of Biotech Wheat for European Agriculture?

            Agnès E. Ricroch

3. Overview of the Wheat Genetic Transformation and Breeding Status in China

            Jiapeng Han, Xiaofen Yu, Junli Chang, Guangxiao Yang, and Guangyuan He

4. Wheat Improvement in India: Present and Future

            Sonia Goel, Kalpana Singh, and N.K. Singh

5. Overview of Methods for Assessing Salinity and Drought Tolerance of Transgenic Wheat Lines

            Rohit Joshi, Khalid Anwar, Priyanka Das, Sneh L. Singla-Pareek, and Ashwani Pareek

6. Allergenicity Assessment of Transgenic Wheat Lines In Silico

            Ankita Mishra and Naveen Arora

7. Agribusiness Perspectives on Transgenic Wheat

            Bill Malcolm

Part II: Protocols

8. Agrobacterium-Mediated Transformation of Wheat Using Immature Embryos

9. Biolistic Transformation of Wheat

            Caroline Tassy and Pierre Barret

10. Wheat Genetic Transformation Using Mature Embryos as Explants

            Harsh Chauhan and Paramjit Khurana

11. Targeted Mutagenesis in Hexaploid Bread Wheat Using the TALEN and CRISPR/Cas Systems

            Yanpeng Wang, Yuan Zong, and Caixia Gao

12. Design and Assembly of CRISPR/Cas9 Reagents for Gene Knock-Out, Targeted Insertion, and Replacement in Wheat

            Tomáš Čermák and Shaun J. Curtin

13. Doubled Haploid Transgenic Wheat Lines by Microspore Transformation

            Sachin Rustgi, Nii O. Ankrah, Rhoda A.T. Brew-Appiah, Yue Sun, Weiguo Liu,  and Diter von Wettstein

14. Doubled Haploid Laboratory Protocol for Wheat Using Wheat-Maize Wide Hybridization

            Meenakshi Santra, Hong Wang, Scott Seifert, and Scott Haley

15. Real-Time PCR for the Detection of Precise Transgene Copy Number in Wheat

            Angelica Giancaspro, Agata Gadaleta, and Antonio Blanco

16. Endogenous Reference Genes and Their Quantitative Real-Time PCR Assays for Genetically Modified Bread Wheat (Triticum aestivum L.) Detection

17. Phenotypic Characterization of Transgenic Wheat Lines Against Fungal Pathogens Puccinia triticina and Fusarium graminearum

            Jagdeep Kaur, Dilip Shah, and John Fellers

18. Databases for Wheat Genomics and Crop Improvement

            Yuxuan Yuan, Armin Scheben, Chon-Kit Kenneth Chan, and David Edwards

19. High Density SNP Genotyping Array for Hexaploid Wheat and Its Relatives

            Amanda J. Burridge, Mark O. Winfield, Alexandra M. Allen, Paul A. Wilkinson, Gary L.A. Barker, Jane Coghill, Christy Waterfall, and Keith J. Edwards

Includes cutting-edge techniques for the study of biotechnological approaches to wheat production

Provides step-by-step detail essential for reproducible results

Contains key implementation advice from the experts