Techniques for Biochemical Analysis
Auteurs : Hussain Khursheed, Lone Sameena Maqbool, Masoodi Khalid Z., Balkhi Syed Minu
Techniques for Biochemical Analysis provides researchers with a practical guide for investigating a variety of different biomolecules. It includes a range of tried and tested protocols, outlining the principles upon which each technique is founded, as well as providing instructions on equipment setup and use, buffer preparation, reagents required, safety considerations and analysis of findings. Beginning with an introduction to biochemistry and laboratory procedures, the book moves on to specific methods focused on investigation of carbohydrates, proteins, enzymes, plant hormones, minerals, amino acids and more. The large range of protocols covered in this foundational how-to reference are interdisciplinary and adaptable to a variety of areas, making this an ideal resource for researchers across various fields, including biochemistry, molecular biology, medical sciences, plant physiology, agriculture, and related subjects.
2. Introduction to laboratory procedures
3. Preparation of solutions and buffers
4. Qualitative analysis of carbohydrates
5. Qualitative analysis of proteins
6. pH (negative logarithm of hydrogen ion concentration)
7. Spectrophotometry
8. Carbohydrates
9. Amino acids and proteins
10. Enzyme assays
11. Vitamin assays
12. Phenolics
13. Plant pigments
14. Plant
15. Antinutritional factors in plants
16. Toxins
17. Extraction and identification of polyacetylenes
18. Alkaloids
19. Plant hormones
20. Immunochemical methods
21. Nitrogenase activity
22. Chlorophyll
23. Proximate analysis
24. Minerals
25. Antinutritional factors in plants
26. Antioxidants
27. Lipids
Sameena Maqbool Lone is a PhD scholar in the Division of Vegetable Science, Sher-e-Kashmir University of Agricultural Sciences and Technology, India. She holds a Master's degree in Horticulture from SKUAST-K, Shalimar. At the Master's level, she worked on "Heterosis and Combining Ability Studies in Cherry Tomato" and obtained competence in morphological and molecular characterization, as well as numerous plant tissue culture technologies. In her Phd programme, she is working on the “Morpho-Molecular Characterization and Nutraceutical Profiling of different carrot genotypes (Daucus carota L.). She has outstanding academic and extracurricular achievements, for which she received a merit scholarship from SKUAST-K during her Bachelor's and Master's degree programmes. She has contributed to over 23 academic papers, 6 books, and more than 100 new gene sequences in Genbank by 2022.
Dr. Khalid Zaffar Masoodi is a young scientist who works as an Assistant Professor in the Division of Plant Biotechnology at the Sher-e Kashmir University of Agricultural Sciences and Technology in Kashmir (India). He holds a Master's degree in Biotechnology and a doctorate in Plant Biotechnology. Dr. Masoodi is the recipient of numerous awards, including the SERB Early Career Research Award 2016, the SBUR Travel Award in the United States of America in 2012, and the Society of Endocrinology Journal Award in 2018 at Glasgow United Kin
- Features step-by-step methods for biochemical analysis of a variety of compounds
- Explores methods that are applicable and adaptable across a variety of fields, including biochemistry, molecular biology, and related areas
- Provides detailed instructions on how to prepare buffers, equipment to be used and analysis of a variety of molecules, including carbohydrates, lipids, proteins, and hormones
- Contains interdisciplinary and adaptable methods and techniques
Date de parution : 04-2024
Ouvrage de 256 p.
Mots-clés :
Qualitative analysis; carbohydrates; proteins; spectrophotometry; amino acids; assay; enzymes; hormones; in vitro methods; lipids
