Principles of Cloning (3^rd Ed.)

Principles of Cloning, Third Edition is the fully revised edition of the authoritative book on the science of cloning. The book presents the basic biological mechanisms of how cloning works and progresses to discuss current and potential applications in basic biology, agriculture, biotechnology, and medicine. Beginning with the history and theory behind cloning, the book goes on to examine methods of micromanipulation, nuclear transfer, genetic modification, and pregnancy and neonatal care of cloned animals. The cloning of various species - including mice, sheep, cattle, and non-mammals is considered as well.

The Editors have been involved in a number of breakthroughs using cloning technique, including the first demonstration that cloning works in differentiated cells done by the Recipient of the 2012 Nobel Prize for Physiology or Medicine - Dr John Gurdon; the cloning of the first mammal from a somatic cell - Drs Keith Campbell and Ian Wilmut; the demonstration that cloning can reset the biological clock - Drs Michael West and Robert Lanza; the demonstration that a terminally differentiated cell can give rise to a whole new individual - Dr Rudolf Jaenisch and the cloning of the first transgenic bovine from a differentiated cell - Dr Jose Cibelli. The majority of the contributing authors are the principal investigators on each of the animal species cloned to date and are expertly qualified to present the state-of-the-art information in their respective areas.

1. Cloning History and Universal Principles

Part I Basics
2. Multiplying Embryos: Experimental Monozygotic Polyembryony in Mammals and its uses
3. Artificial Activation of Mammalian Oocytes
4. Nuclear Origins and Clone Phenotype
5. Genetic and Phenotypic Similarity Among Members of Mammalian Clonal Sets

Part II Methods
6. Micromanipulation Techniques for Cloning
7. Handmade Cloning
8. Culture of Viable Mammalian Embryos In Vitro
9. Pregnancy and Neonatal Care of SCNT Animals
10. Donor Cell Type and Cloning Efficiency in Mammals
11. Enhancing SCNT with Chromatin Remodeling Agents
12. Cell Cycle Regulation in Cloning

Part III Cloning by Species
13. Amphibia
14. Medaka Fish
15. Zebrafish
16. Goldfish
17. Mice
18. Rabbits
19. Pigs
20. Cows
21. Dogs
22. Equids
23. Macaque Monkeys

Part IV Complementary Technologies
24. Genome Editing in Somatic Cells
25. Targeted Chromosome Elimination from ES-Somatic Hybrid Cells

Part V Applications
26. Nuclear Transfer for Stem Cells
27. Embryonic Stem Cell in Bovine (other species?)
28. Commercial Applications of Cloning Technology
29. Production of Recombinant Therapeutic Proteins
30. Generation of Transgenic Domestic Species via Somatic Cell Nuclear Transfer
31. Commercializing Genetically Engineered Cloned Cattle
32. Cloning Endangered Species
33. Genetically Engineered Pigs for Medicine

Part VI Mechanisms
34. Histone Methylation During Somatic Cell Nuclear Transfer
35. Nuclear Transfer with Germ Cells: Germ Cell Cloning Contributes to current Understanding of Genomic Imprinting in Mammals
36. Clone-Specific X-Linked Gene Repression Caused by Ectopic Xist Transcripts from the Active X Chromosome
37. Cell Division and Nuclear Reprogramming
38. Reprogramming Machinery of the Mouse Oocyte – Proteomic Analyses
39. Biological Age of Cloned Animals
40. Mitochondrial Inheritance in Cloned Animals
41. Interspecies Somatic Cell Nuclear Transfer
42. Epigenetics of Cloned Livestock Embryos and Offspring
43. Role of iPSC-Producing Factors in Pre-Implantation Embryos
44. Chromatin architecture reorganization in murine somatic cell nuclear transfer embryos
45. Genome Exchange in Human Oocytes

Part VII Ethical, Regulatory and Legal Affairs
46. Ethical Implications of Reproductive Cloning
47. An Overview of the Regulatory Considerations for Animal Cloning in the USA
48. Regulation and safety considerations of somatic cell nuclear transfer-cloned farm animals and their offspring used for food production – the European Union perspective
49. Asian Regulatory Landscape on Animal Cloning
50. South American Governmental guidelines for the production and commercialization of Cloned Animals

Part VIII Cloning Outlook
51. Somatic cell nuclear transfer: failures, successes, and the challenges ahead

Dr. Cibelli is Professor of Animal Biotechnology at Michigan State University. He heads the Cellular Reprogramming Laboratory in the Departments of Animal Science and Physiology since 2003. From 2010 to 2017 he was also the Scientific Director of LARCel, a laboratory of cellular reprogramming dedicated to generating human pluripotent cells under GMP conditions for preclinical studies in Andalucia, Spain. Dr. Cibelli is internationally recognized as one of the pioneers in the area of cellular reprogramming using oocyte-driven protocols. Dr. Cibelli together with his colleagues, were responsible for the generation of the world's first transgenic cloned calves, the first stem cells by nuclear transfer in bovine, the first embryonic stem cells by parthenogenesis in primates and the generation of the first cell line of iPSCs using oocyte factors alone. His work has been published un numerous scientific journals including Science, Nature Biotechnology, Nature Medicine, Nature Methods, PNAS, Cell Stem Cell and JAMA.