Handbook of Translational Transcriptomics Research, Protocols and Applications

Langue : Anglais

Coordonnateur : Buzdin Anton

Résumé
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Handbook of Translational Transcriptomics: Research, Protocols and Applications provides a comprehensive overview to the field of transcriptomics. With an emphasis on the various protocols and techniques available for investigation, it acts as a practical guide to researchers for implementing their own investigations in the field. The book begins with an overview of the past, present, and potential approaches in the field of transcriptomics, with discussion of choosing the correct approach based on the research needed, it also highlighting the pros and cons of each approach. Following this it explores techniques and protocols for investigating specific approaches focusing on RNA sequencing, expression arrays, and gene expression, providing detailed. It then delves into data analysis and offers recommendations, guidelines, and approaches related to data interpretation. The book also considers the translation of transcriptomics to clinical application and applications in molecular diagnostics, biomarkers in medicine, and personalized medicine specific to oncology, as well as biotechnology for pharmaceutical research. Handbook of Translational Transcriptomics is a detailed reference that provides a complete view of transcriptomics, ranging from methods to handling data and medical applications.

1. Past, current and future of transcriptomics 2. Pitfalls of transcriptomics, and selection of the most appropriate transcriptomic technique 3. RNA sequencing in wet lab 4. Expression arrays in wet lab 5. High-throughput and multiplex PCR in wet lab to measure gene expression 6. Processing primary gene expression data: normalization, harmonization and data QC 7. Data check and comparison with the expression databases 8. Finding differentially expressed genes and gene sets 9. Molecular pathway analysis using transcriptomic data 10. OMICSwise analysis of RNA splicing 11. Transcriptomic biomarkers in medicine 12. Translational transcriptomics for personalized oncology 13. Transcriptomics for modern biotechnology 14. Transcriptomics and quantitative proteomics: competition or symbiosis?

Anton Buzdin is professor and head of laboratories at Sechenov First Moscow Medical University, Moscow Institute of Physics and Technology, and Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry. He was elected as the chair of Biostatistics and Bioinformatics subgroup, Pathobiology Group of European Organization for Research and Treatment of Cancer (EORTC). Professor Buzdin is cofounder and chief scientific officer of Oncobox, which is a translational oncology company. He has authored more than 170 scientific papers and patent applications, and has worked as chief executive of several biotechnological and biomedical companies in Russia, Hong Kong and in the United States. He has also chaired two clinical trials of transcriptomics-based diagnostic tools in oncology.
Professor Buzdin’s team pioneered bioinformatic quantitative analysis of molecular pathways using gene expression data and developed specialized algorithms and software packages to personalize prescription of targeted immunotherapy and chemotherapy drugs to cancer patients that were clinically validated and commercialized.

Features practical guidance and protocols for researchers to replicate transcriptomic methods and techniques, including wet lab techniques
Includes useful information on analyzing and interpreting transcriptomic data
Offers a detailed introduction into translational transcriptomics, exploring both coding and non-coding RNAs including microRNA.
Investigates clinical implications of transcriptomics and applications to treating disease
Considers some experimental and bioinformatic techniques in the field of transcriptomics, as well as more established approaches