Enzyme assays : High-throughout screenin g, genetic selection & fingerprinting

Edited by one of the leading experts in the field, this book fills the need for a book presenting the most important methods for high-throughput screenings and functional characterization of enzymes. It adopts an interdisciplinary approach, making it indispensable for all those involved in this expanding field, and reflects the major advances made over the past few years. For biochemists, analytical, organic and catalytic chemists, and biotechnologists.

THE CHEMISTRY OF ENZYME ASSAYS. Chromogenic Assay of Hydrolases for Activity and Enantioselectivity. High-throughput screening systems for assaying the enantioselectivity of enzymes. High throughput screening methods developed for oxidoreductases. Industrial perspective on assays. ENZYME ASSAYS AND GENETIC SELECTION. Agar-plate based assays. Genetic selection for biocatalysts. Three-hybrid systems and in vivo selection. Nucleic-acid based approaches on the screening of enzymes. ENZYME PROFILING. Fluorescent probes for lipolytic enzymes. Enzyme fingerprinting. Protease profiling. Enzyme assays on chips. INDEX .