Cryo-electron Tomography A Journey from Sample Preparation to Data Mining
Coordonnateurs : Hanein Dorit, Volkmann Niels
Part I: Sample Preparation 1. VLP (Virus-like particles) 2. Viruses 3. Bacteria 4. n situ cellular tomography 5. Correlative techniques 6. Host pathogen interactions 7. Towards organoids and tissues Part II: Data Mining 1. Sub-tomogram averaging 2. Segmentation 3. Denoising 4. Feature detection 5. Validation
Niels Volkmann earned his Ph.D. in Biophysics from the University of Hamburg, Germany in 1993, where he also trained as a postdoctoral fellow at the Max Planck Research Unit for Ribosome Structure. He received additional postdoctoral training at Brandeis University at the Rosenstiel Basic Medical Science Research Center and The Keck Center for Cellular Visualization prior to joining Sanford Burnham Prebys in 1999. Dr. Volkmann was promoted to the faculty as Assistant Professor in 2001.
Dr. Volkmann’s lab seeks to understand biological processes as integrated, hierarchical systems rather than as isolated parts, by developing and applying quantitative analysis tools to link and integrate the scales of atomic-resolution information and imaging of dynamic events in living cells. A key feature of Dr. Volkmann’s research is the highly synergistic collaborations between his laboratory and other research groups (particularly Dr. Hanein’s) that are addressing fundamental questions in cell and cancer biology.
- Provides a detailed overview of cryo-ET sample preparation and data mining protocols and approaches in a single resource
- Includes foundational knowledge underpinning the field
- Explores the latest methods, techniques and recent developments in cryo-ET
- Offers step-by-step guidance to researchers for key procedures
- Written by an international range of experts
Date de parution : 11-2024
Ouvrage de 360 p.
15.2x22.8 cm