Rheumatoid Arthritis (2^nd Ed., 2nd ed. 2024) Methods and Protocols Methods in Molecular Biology Series, Vol. 2766

This second edition explores standard laboratory protocols and methodology commonly used in basic and translational studies in the field of rheumatoid arthritis (RA) treatment. Chapters detail including basic RA models, evaluation of disease activity and immunological status, systemic drug delivery, and new research tools. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Rheumatoid Arthritis: Methods and Protocols,  Second Edition aims to be a basic manual for clinical researchers who are just getting started in the field of intervention study. 

Part I Animal Models

1. Collagen-induced arthritis models

Miyoshi Maya and Shuang Liu

2. Human xenograft model

Shuang Liu

3. Scaffolded chondrogenic spheroid-engrafted model

Shuang Liu

4. Denervation-induced sarcopenia model

Erika Takemasa and Shuang Liu

5. Long-term constant subcutaneous drug administration

6. Clinical Scoring of Disease Activity in Animal Models

Maya Miyoshi and Shuang Liu

7. Histological analyses of arthritic joints in collagen-induced arthritis model mice

Takeshi Kiyoi

8. Preparation of Joint Extracts

Shuang Liu and Erika Takemasa

Part II Therapeutic Approach

9. Production of immunizing antigen proteoliposome using cell-free protein synthesis system

Wei Zhou and Hiroyuki Takeda

10. Re-construction of protein/liposome complex

Yasuyuki Suzuki

11. Production of neutralizing antibody

Erika Takemasa and Shuang Liu

12. Autoantibody profiling using human autoantigen protein array and AlphaScreen

Hiroyuki Takeda

13. Generation of Specific Aptamers

Shuang Liu, Yasuyuki Suzuki, and Makoto Inui

Yasuyuki Suzuki

15. RNA interference ex vivo

Shuang Liu

Shuang Liu

17. Lentiviral production platform

Shuang Liu

18. Mesenchymal stem cell engineering

Shuang Liu

Part III Evaluation of Immunological status

19. Screening of Ca²⁺ influx in lymphocytes

Erika Takemasa and Shuang Liu

20. Single-cell Ca²⁺ imaging

21. Electrophysiological methods to measure Ca²⁺ current

Shuang Liu

22. Evaluation of mitochondrial respiratory function in murine splenocytes

Mochitsuki Marii and Shuang Liu

23. The Functional Assessment of T Cells

Saho Maruyama

24. Release of antibodies and cytokines from B cells

Shuang Liu

25. Evaluation of autoreactive responses

Shaung Liu

26. Macrophage polarization and osteoclast differentiation

Noritaka Saekiand Akihiro Nakata

27. Scanning electron microscopic analysis of the bone-resorption activity in mature osteoclasts

Takeshi Kyoi

28. Animal Models of Vasculitis

Masaki Mogi and Shuang Liu

Takeshi Kiyoi

30. Bulk RNA-seq assessment of murine spleen  using a portable MinION sequencing device

Yasuyuki Suzuki and Shuang Liu

Part IV Clinical approach

31. Institutional review board considerations for clinical trials

Masaki Mogi and Shuang Liu

32. Design an intervention study

Jun Ishizaki and Hitoshi Hasegawa

33. Assessment of disease activity, structural damage, and function in rheumatoid arthritis

Jun Ishizaki and Hitoshi Hasegawa

Jun Ishizaki

35. 16S rRNA gene amplicon analysis of human gut microbiota

Noriyuki Miyaue

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts