Xylem (2^nd Ed., 2nd ed. 2024) Methods and Protocols Methods in Molecular Biology Series, Vol. 2722

This second edition provides detailed techniques used for the study and characterization of the plant vascular system, with a central focus on the xylem tissue.  Chapters are organized in three main sections covering; analysis of xylem development, xylem characterization though imaging techniques, and analysis of the xylem composition. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Xylem: Methods and Protocols, Second Edition aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge. 

 Part I: xylem transport, functional dynamics and modelling

1. Monitoring Xylem Transport in Arabidopsis thaliana Seedlings Using Fluorescent Dyes

Kai Bartusch, Noel Blanco-Touriñán, Antia Rodriguez-​Villalon, and Elisabeth Truernit

Anita Roth-Nebelsick and Wilfried Konrad

3. Modeling Xylem Functionality Aspects

Alex Tavkhelidze, Gerhard Buck-Sorlin, and Winfried Kurth

4. Detecting and quantifying xylem embolism by synchrotron-based x-ray micro-CT

Martina Tomasella, Francesco Petruzzellis, Sara Natale, Giuliana Tromba,  and Andrea Nardini

Part II: xylem development and evolution

5. Analysis of xylem cells by nucleus-based transcriptomics and chromatin profiling

Dongbo Shi, Laura Luzzietti, Michael Nodine, and Thomas Greb

6. Quantification of xylem-specific thermospermine-dependent translation of SACL transcripts with dual luciferase reporter system

Anna Solé-Gil, Cristina Úrbez, Alejandro Ferrando, and Miguel A. Blázquez

7. Fossil wood analyses: several examples from five case studies in the area of Central and NW Bohemia, Czech Republic

Jakub Sakala

Part III: xylem diseases

8. Isolation and reproductive structures induction of fungal pathogens associated with xylem and wood necrosis in grapevine

Ana López-Moral and Carlos Agustí-Brisach

9. Determination of de novo suberin-lignin ferulate deposition in xylem tissue upon vascular pathogen attack

Weiqi Zhang, Álvaro Jiménez-Jiménez, Montserrat Capellades, Jorge Rencoret, Anurag Kashyap, and Núria S. Coll

Part IV: xylem composition and imaging

10. Quantification of treacheary elements types in mature hypocotyl of Arabidopsis thaliana

Paula Brunot-Garau, Cristina Urbez, and Francisco Vera-Sirera

Marta-Marina Pérez Alonso, Àngela Carrió-Seguí, and Hannele Tuominen

12. Lignin analysis by HPLC and FTIR: spectra deconvolution and S/G ratio determination

13. Inducible pluripotent suspension cell cultures (iPSCs) to study plant cell differentiation

Delphine Ménard, Henrik Serk, Raphael Decou, and Edouard Pesquet

Edouard Pesquet, Leonard Blaschek, Junko Takahashi, Masanobu Yamamoto, Antoine Champagne, Nuoendagula, Elena Subitona, Charilaos Dimotakis, Zoltan Bascik, and Shinya Kajita

15. Clearing of vascular tissue in Arabidopsis thaliana for reporter analysis of gene expression

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts