Plastids (2nd Ed., 2nd ed. 2024) Methods and Protocols Methods in Molecular Biology Series, Vol. 2776
Coordonnateur : Maréchal Eric
This second edition volume expands on the previous edition with new and updated chapters that explore our current view on plastid evolution, structure, and function. The chapters in this book are organized into three parts and cover topics such as plastid evolution, plasticity, and functional and structural diversity; techniques used to visualize, fractionate, purify, and study primary plastids from plant materials, and secondary plastids; and methods to analyze plastids by integrated biology strategies based on genetics, genomics, proteomics, and lipidomics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and authoritative, Plastids: Methods and Protocols, Second Edition is a valuable resource that will help students, engineers, and researchers further explore and understand this fascinating organelle.
Preface…
Table of Contents…
Contributing Authors…
Part I Plastid Evolution, Plasticity, Structural, and Functional Diversity
1. How Did Thylakoids Emerge in Cyanobacteria and How Were the Primary Chloroplast and Chromatophore Acquired?
Eric Maréchal
2. Complex Endosymbioses I: From Primary to Complex Plastids, Serial Endosymbiotic Events
Zoltán Füssy and Miroslav Obornik
3. Complex Endosymbiosis II: The Non-Photosynthetic Plastid of Apicomplexa Parasites (The Apicoplast) and Its Integrated Metabolism
Nyamekye Quansah, Charital Sarah, Yoshiki Yamaryo-Botté, and Cyrille Y. Botté
4. Diversification of Plastid Structure and Function in Land Plants
Henrik Aronsson and Katalin Solymosi
5. The Main Functions of Plastids
Marcel Kuntz, Laura Dimnet, Sara Pullara, Lucas Moyet, and Norbert Rolland
6. Plastid Transient and Stable Interactions with Other Cell Compartments
Stefanie J. Mueller-Schuessele, Sébastien Leterme, and Morgane Michaud
Part II Laboratory Protocols
7. Purification of Chloroplast Envelope, Thylakoids, and Stroma from Angiosperm Leaves
Maryse A. Block, Catherine Albrieux, and Eric Maréchal
8. Isolation of the Inner and Outer Membranes of the Chloroplast Envelope from Angiosperms
Maryse A. Block and Eric Maréchal
9. Analysis of a Super-Complex at Contact Sites between Mitochondria and Plastids
Morgane Michaud
10. Isolation of High-Quality Plastids from the Diatom Phaeodactylum tricornutum
Fan Hu, Wenxiu Yin, Teng Huang, and Hanhua Hu
11. Determining the Subcellular Localization of Proteins in the Different Membranes of Diatom Secondary Plastid
Xiaojuan Liu and Yangmin Gong
12. Monitoring of Lipid Fluxes between Host and Plastid-Bearing Apicomplexan Parasites
Sarah Charital, Amandine Lourdel, Nymekye Quansah, Cyrille Y. Botté, and Yoshiki Yamaryo-Botté
13. Extraction and Quantification of Lipids from Plant or Algae
Valérie Gros, Josselin Lupette, and Juliette Jouhet
14. Quantitative Assessment of the Chloroplast Lipidome
Valérie Gros and Juliette Jouhet
15. The Use of Nanopore Sequencing to Analyze the Chloroplast Transcriptome Part I: Library Preparation
Sébastien Skiada, Alexandra Launay-Avon, Arnaud Liehrmann, Etienne Delannoy, and Benoît Castandet
16. The Use of Nanopore Sequencing to Analyze the Chloroplast Transcriptome Part II: Bioinformatic Analyzes and Virtual RNA Blots
Etienne Delannoy, Arnaud Liehrmann, and Benoît Castandet
17. Targeted Gene Editing of Nuclear-Encoded Plastid Proteins in Phaeodactylum tricornutum via CRISPR/Cas9
Cécile Giustini, Jhoanell Angulo, Florence Courtois, and Guillaume Allorent
18. Isolation of Cytosolic Ribosomes Associated with Plant Mitochondria and Chloroplasts
Laura Dimnet, Thalia Salinas-Giegé, Sara Pullara, Lucas Moyet, Chloé Genevey, Marcel Kuntz, Anne-Marie Duchêne, and Norbert Rolland
Part III In Silico Tools
Gilles Curien
Subject Index List…
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible result
Contain key notes and implementation advice from the experts
Date de parution : 05-2024
Ouvrage de 322 p.
17.8x25.4 cm
Thème de Plastids :
Mots-clés :
Apicomplexa; cyanobacteria; Primary endosymbioses; thylakoids; electron microscopy
