Micropropagation Methods in Temporary Immersion Systems, 1st ed. 2024 Methods in Molecular Biology Series, Vol. 2759

Part I: Temporary Immersion System Designs and Applications

1. Temporary Immersion Systems in Plant Micropropagation

            Marco A. Ramírez-Mosqueda and Carlos A. Cruz-Cruz

2. Types of Temporary Immersion Systems Used in Commercial Plant Micropropagation

            Ivonne N. Bravo-Ruíz, Ma. Teresa González-Arnao, Fabiola Hernández-Ramírez, Jaime López-Domínguez, and Carlos A. Cruz-Cruz

3. Use of Temporary Immersion Systems in the Establishment of Biofactories

            Marco Vinicio Rodríguez-Deméneghi

Part II: Micropropagation in TIS of Agroindustrial Species: Protocols

4. Large-Scale Micropropagation of Vanilla (Vanilla planifolia Jacks.) in a Temporary Immersion Bioreactor (TIB)

            Marco A. Ramírez-Mosqueda, Marco Vinicio Rodríguez-Deméneghi, Heidi P. Medorio-García, and Rubén H. Andueza Noh

5. Temporary Immersion Bioreactors for Sugarcane Multiplication and Rooting

            José Luis Spinoso-Castillo, María Karen Serrano-Fuentes, Monserrat Sorcia-Morales, and Jericó Jabín Bello-Bello

6. Micropropagation of Stevia (Stevia rebaudiana Bert.) in RITA®

            Heidi P. Medorio-García, Elizabeta Hernández-Domínguez, Rubén H. Andueza Noh, David R. Lopez-Aguilar, and Marco A. Ramírez-Mosqueda

7. In Vitro Multiplication of Agave (A. marmorata and A. potatorum) by Temporary Immersion in SETIS™ Bioreactor

            María del Rosario Moreno-Hernández, Eucario Mancilla-Álvarez, Daniel Aguilar-Jiménez, and Jericó Jabín Bello-Bello

8. BioMINT: A Temporary Immersion System for Agave Micropropagation

            Kelly M. Monja-Mio, Gabriel Ojeda, Miguel Ángel Herrera-Alamillo, Lorenzo Felipe Sánchez-Teyer, and Antonio Rescalvo-Morales

9. Plant Regeneration of Agave cupreata by Somatic Embryogenesis in a Temporary Immersion System with Silver Nanoparticles

            Sandra Y. Martínez-Martínez, Amaury M. Arzate-Fernández, and María G. Gonzáles-Pedroza

10. Micropropagation of Chayote (Sechium edule L.) var. virens levis in RITA®

            Lizandro Ramírez-Trejo, Rosalía Núñez-Pastrana, and Anell Soto-Contreras

Part III: Micropropagation in TIS of Ornamental Species: Protocols

11. Direct Shoot from Root and True-to-Type Micropropagation of Limonium ‘Misty Blue’ in Partially Immersed Culture on an Aluminum Mesh Raft

            Priyanka Raha, Gourab Saha, Ishita Khatua, and Tapas Kumar Bandyopadhyay

12. Increased Multiplication Rates of Vriesea hieroglyfica (Carriere) E. Morren through a Temporary Immersion System

            Carolina Rossi de Oliveira, Alice Noemi Aranda-Peres, Leonardo Soriano, Paulo Hercílio Viegas Rodrigues, and Adriana Pinheiro Martinelli

13. Micropropagation of Encyclia cordigera (Kunth) Dressler in Ebb-and-Flow Bioreactor

            Obdulia Baltazar-Bernal and Evelia Guadalupe Mora-González

14. Micropropagation of Guarianthe skinneri (Bateman) Dressler & W. E. Higging in Temporary Immersion Bioreactors

            Elizabeta Hernández-Domínguez, David R. Lopez-Aguilar, Andrés Orduño-Cruz, Pedro Zetina-Córdoba, and Marco A. Ramírez-Mosqueda

15. Alstroemeria Micropropagation in a RITA® Temporary Immersion System

            Lilia Castro Pereira, Leonardo Soriano, Carolina Rossi de Oliveira, Paulo Hercílio Viegas Rodrigues, and Adriana Pinheiro Martinelli

Part IV: Micropropagation in TIS of Woody Species: Protocols

16. Proliferation of Axillary Shoots of Chestnut in Temporary Immersion Systems

            Nieves Vidal, Conchi Sánchez, and Beatriz Cuenca

17. In Vitro Propagation of Plants via Organogenesis in Bambusa vulgaris Schrad. ex Wendl Using Temporary Immersion Systems

            Yudith García-Ramírez, Mallelyn González-González, Marisol Freire-Seijo, Raúl Barbón-Rodríguez, and Sinesio Torres-García

18. Multiplication of Cupressus guadalupensis Using the RITA® Temporary Immersion System

            Luis Alberto Gómez-Reyes, Esmeralda Judith Cruz-Gutiérrez, Lorena Jacqueline Gómez-Godínez, Manuel de Jesús Bermúdez-Guzmán, Claudia Berenice Espitia-Flores, and Juan Manuel Pichardo González

19. Secondary Embryogenesis of Linaloe in Temporary Immersion Bioreactors TypeRITAÒ

            Gregorio Arellano-Ostoa, Mónica González-Orozco, Izaac Vázquez-Cisneros, and Sandra M. Arellano-González

Part V: Reviews of the Use of Temporary Immersion Systems in Micropropagation

20. Temporary Immersion System for Biomass Production of Salvia spp: A Mini-Review

            Yudith García-Ramírez

21. Orchid Micropropagation Using Temporary Immersion Systems: A Review

            Obdulia Baltazar-Bernal, Evelia Guadalupe Mora-González, and Marco A. Ramírez-Mosqueda

Part VI: General Conclusions and Perspectives

22. Conclusions and Perspectives on Plant Micropropagation in Temporary Immersion Systems

            Marco A. Ramírez-Mosqueda and Carlos A. Cruz-Cruz

Includes cutting-edge techniques

Provides step-by-step detail essential for reproducible results

Contains key implementation advice from the experts